In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1

Dengue disease affects 50-100 million people every year worldwide. In Argentina, three serotypes circulate, with a major prevalence of DENV-1 (72%). Effective antiviral drugs are not currently available. Medicinal plants may contain bioactive principles capable of inhibiting the replication of the d...

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Autores principales: Peña, VS, Luque, FG, Soria, EA, Sabini, MC
Formato: Artículo revista
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022
Materias:
Dengue Virus
Antiviral Agents
Arachis
Phytotherapy
Virus del Dengue
Antivirales
Fitoterapia
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/39056
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-39056
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
format Artículo revista
topic Dengue Virus
Antiviral Agents
Arachis
Phytotherapy
Virus del Dengue
Antivirales
Arachis
Fitoterapia
spellingShingle Dengue Virus
Antiviral Agents
Arachis
Phytotherapy
Virus del Dengue
Antivirales
Arachis
Fitoterapia
Peña, VS
Luque, FG
Soria, EA
Sabini, MC
In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1
topic_facet Dengue Virus
Antiviral Agents
Arachis
Phytotherapy
Virus del Dengue
Antivirales
Arachis
Fitoterapia
author Peña, VS
Luque, FG
Soria, EA
Sabini, MC
author_facet Peña, VS
Luque, FG
Soria, EA
Sabini, MC
author_sort Peña, VS
title In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1
title_short In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1
title_full In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1
title_fullStr In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1
title_full_unstemmed In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1
title_sort in vitro evaluation of the antiviral properties of arachis hypogaea l. extracts against dengue virus serotype 1
description Dengue disease affects 50-100 million people every year worldwide. In Argentina, three serotypes circulate, with a major prevalence of DENV-1 (72%). Effective antiviral drugs are not currently available. Medicinal plants may contain bioactive principles capable of inhibiting the replication of the dengue virus, with Arachis hypogaea L. (peanut) standing out for its biological properties. The aim of the present work was to evaluate the antiviral activity of Tegument ethanolic extract (TEE) and Seed ethanolic extract (SEE) of peanuts against DENV-1 on Vero cells. TEE and SEE were obtained by simple alcoholic extraction. The 50% cytotoxic concentration (CC50) on Vero cells was determined by Neutral Red Uptake (NRU) and MTT assays at 7 days. Antiviral activity was then assessed throughout the replication cycle using the lysis plate reduction technique. Cell monolayers were infected with 50 PFU/well of DENV-1, and treated with TEE (30μg/mL) and SEE (300μg/mL) during adsorption and penetration and, in intracellular stages of replication, incubated 7 days at 37°C. Subsequently, the cells were fixed with formaldehyde solution (10%) and stained with violet crystal (1%). Lysis plates were counted and viral inhibition was determined. Antiviral action was then evaluated at different stages of the replication cycle using the same technique. Statistical analysis (t-test and ANOVA, p<0.05) was performed using Graph Pad Prism 6.0. The CC50 values were 169μg/mL and 65μg/mL for TEE and >1400μg/mL and 636μg/mL for SEE by NRU and MTT, respectively. The study of antiviral activity throughout the cycle of replication showed that TEE inhibited DENV-1 (92.5±0.9%) (p<0.001). The study of the target of action indicated that TEE acted in stages of viral adsorption and penetration (92.5±0.9%) but did not act in stages post-penetration (15.7±6.6%). Also, it exerted virucidal action (98±5.0.7%) and inhibited the virus (78±3.04%) in cellular pre-treatment (p<0.001). On the other hand, the SEE did not show inhibitory capacity against DENV-1 (23.1±2.9%). In conclusion, the data obtained demonstrates the potential application of tegument extract from peanut in the development of an antiviral phytotherapeutic against the dengue virus.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2022
url https://revistas.unc.edu.ar/index.php/med/article/view/39056
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first_indexed 2024-09-03T21:04:05Z
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spelling I10-R327-article-390562024-04-15T16:14:45Z In vitro evaluation of the antiviral properties of Arachis hypogaea L. extracts against dengue virus serotype 1 Evaluación in vitro de las propiedades antivirales de extractos de Arachis hypogaea L. contra el virus del dengue serotipo 1 Peña, VS Luque, FG Soria, EA Sabini, MC Dengue Virus Antiviral Agents Arachis Phytotherapy Virus del Dengue Antivirales Arachis Fitoterapia Dengue disease affects 50-100 million people every year worldwide. In Argentina, three serotypes circulate, with a major prevalence of DENV-1 (72%). Effective antiviral drugs are not currently available. Medicinal plants may contain bioactive principles capable of inhibiting the replication of the dengue virus, with Arachis hypogaea L. (peanut) standing out for its biological properties. The aim of the present work was to evaluate the antiviral activity of Tegument ethanolic extract (TEE) and Seed ethanolic extract (SEE) of peanuts against DENV-1 on Vero cells. TEE and SEE were obtained by simple alcoholic extraction. The 50% cytotoxic concentration (CC50) on Vero cells was determined by Neutral Red Uptake (NRU) and MTT assays at 7 days. Antiviral activity was then assessed throughout the replication cycle using the lysis plate reduction technique. Cell monolayers were infected with 50 PFU/well of DENV-1, and treated with TEE (30μg/mL) and SEE (300μg/mL) during adsorption and penetration and, in intracellular stages of replication, incubated 7 days at 37°C. Subsequently, the cells were fixed with formaldehyde solution (10%) and stained with violet crystal (1%). Lysis plates were counted and viral inhibition was determined. Antiviral action was then evaluated at different stages of the replication cycle using the same technique. Statistical analysis (t-test and ANOVA, p<0.05) was performed using Graph Pad Prism 6.0. The CC50 values were 169μg/mL and 65μg/mL for TEE and >1400μg/mL and 636μg/mL for SEE by NRU and MTT, respectively. The study of antiviral activity throughout the cycle of replication showed that TEE inhibited DENV-1 (92.5±0.9%) (p<0.001). The study of the target of action indicated that TEE acted in stages of viral adsorption and penetration (92.5±0.9%) but did not act in stages post-penetration (15.7±6.6%). Also, it exerted virucidal action (98±5.0.7%) and inhibited the virus (78±3.04%) in cellular pre-treatment (p<0.001). On the other hand, the SEE did not show inhibitory capacity against DENV-1 (23.1±2.9%). In conclusion, the data obtained demonstrates the potential application of tegument extract from peanut in the development of an antiviral phytotherapeutic against the dengue virus. La enfermedad del dengue afecta a 50-100 millones de personas cada año mundialmente. En Argentina, circulan tres serotipos, con presencia mayoritaria del DENV-1 (72%). No se dispone actualmente de medicamentos antivirales efectivos. Las plantas medicinales pueden contener principios bioactivos capaces de inhibir la replicación del virus del dengue, destacándose Arachis hypogaea L. (maní) por sus propiedades biológicas. El objetivo de este trabajo fue evaluar la actividad antiviral de los extractos etanólico de tegumento (EET) y semilla (EES) de maní frente a DENV-1 en células Vero. Los EET y EES se obtuvieron mediante extracción alcohólica simple. Se determinó la concentración citotóxica 50% (CC50) en células Vero, mediante captación de rojo neutro (RN) y reducción de MTT, a los 7 días. Luego, se evaluó la actividad antiviral durante todo el ciclo de replicación mediante la técnica de reducción de placas de lisis. Monocapas celulares fueron infectadas con 50 UFP/pocillo de DENV-1, y tratadas con EET (30μg/mL) y EES (300μg/mL) durante la adsorción y penetración y, en etapas intracelulares de replicación, se incubó 7 días a 37°C. Posteriormente, las células se fijaron con formaldehído (10%) y se tiñeron con cristal violeta (1%). Se contaron las placas de lisis y se determinó la inhibición viral. Luego, se evaluó la acción antiviral en diferentes etapas del ciclo de replicación empleando la misma técnica. Se realizó análisis estadístico (t-test y ANOVA, p<0,05) mediante Graph Pad Prism 6.0. Las CC50 fueron 169μg/mL y 65μg/mL  para EET y >1400μg/mL y 636μg/mL para EES por RN y MTT, respectivamente. El estudio de actividad antiviral en todo el ciclo mostró que EET inhibió en un 92,5±0,9% a DENV-1 (p<0,001). El estudio del blanco de acción indicó que EET actuó en etapas de Adsorción-Penetración viral (92,5±0,9%), sin acción en etapas posteriores a la penetración (15,7±6,6%). También, ejerció acción virucida (98,5±0,7%) e inhibió al virus un 78±3,04% en el pre-tratamiento celular (p<0,001). Por otra parte, el EES no mostró capacidad inhibitoria frente a DENV-1 (23,1±2,9%). En conclusión, los datos obtenidos demuestran la potencial aplicación del extracto de tegumento de maní en el desarrollo de un fitoterápico antiviral contra el virus del dengue.  Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2022-10-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion . https://revistas.unc.edu.ar/index.php/med/article/view/39056 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 79 No. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 79 Núm. Suplemento JIC XXIII (2022): Suplemento JIC XXIII Revista da Faculdade de Ciências Médicas de Córdoba; v. 79 n. Suplemento JIC XXIII (2022): Suplemento JIC XXIII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0

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