Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures

During studv on ccli cultures ii'oni total human blood leukocytes, we found a rosettc-shaped ceil association fr,ni o 'd hy,monoeytesand 1 yniphocyw.. Cunsidering the immunoiogic roles of thcse two ccli types, we decided to stiiclv such ccli association, the resuts bcing reported here toge...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Cabral, Humberto, Novak, Ivón
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 1992
Materias:
.
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/45045
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-45045
record_format ojs
spelling I10-R327-article-450452024-11-27T20:43:30Z Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures Cabral, Humberto Novak, Ivón . During studv on ccli cultures ii'oni total human blood leukocytes, we found a rosettc-shaped ceil association fr,ni o 'd hy,monoeytesand 1 yniphocyw.. Cunsidering the immunoiogic roles of thcse two ccli types, we decided to stiiclv such ccli association, the resuts bcing reported here together with data un the Ircquency of thvir odurrence in ('('II cultures of human leukocytes of lx'althy subjccts, at different cultureles.Total ieukocytes were obtained from blood of healthy human donors (9 wornen, 8 unen; mean age 34, rangc 20-62). Venous blood was drained into svringes prcviouslv treated with phenul-Irce hcparin The piasma containing the whitc celis were seedeci in TC 199 medium plus penicilin-strepto- mycin. Samples of cultures were prepared at 48 and 96 hours by slight shaking of the respective flask. The celis were centrifugated at 200 g for 10 minutes and the superlaatant was discarded. The ce'] peliet vas gently resuspended in clrops of TC 199 mediurn. Drops of this cytopre para tion were put Oil gias.s sUdes for 5 min in a humidified charnber, and then dessicated by centrifugation on a disk perpendicular to the axi of the centrifuge, as prcviously described 1 . The cytopreparations were stai- ucd with May Criinwald-Giemsa, and observed at a Iight microscope, at 400 x.Tu considering that the monocytes WC) e found singly and centrally located in the rosettes we have described, the quantification of the rosettes vas made as the percentage of monocytes forming rosettes, by studving 50 of such celis in each case. A rosette was considc'recl when threc oc more lymphocvtes suirroiinded a rnonocytc. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 1992-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/45045 10.31053/1853.0605.v50.n2.45045 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 50 No. 2 (1992); 25-26 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 50 Núm. 2 (1992); 25-26 Revista da Faculdade de Ciências Médicas de Córdoba; v. 50 n. 2 (1992); 25-26 1853-0605 0014-6722 10.31053/1853.0605.v50.n2 spa https://revistas.unc.edu.ar/index.php/med/article/view/45045/45074 Derechos de autor 2024 Universidad Nacional de Córdoba http://creativecommons.org/licenses/by-nc/4.0
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic .
spellingShingle .
Cabral, Humberto
Novak, Ivón
Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures
topic_facet .
author Cabral, Humberto
Novak, Ivón
author_facet Cabral, Humberto
Novak, Ivón
author_sort Cabral, Humberto
title Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures
title_short Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures
title_full Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures
title_fullStr Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures
title_full_unstemmed Spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures
title_sort spontaneous formation of rosettes by autologous human monocyte-macrophages and lymphocytes in cell cultures
description During studv on ccli cultures ii'oni total human blood leukocytes, we found a rosettc-shaped ceil association fr,ni o 'd hy,monoeytesand 1 yniphocyw.. Cunsidering the immunoiogic roles of thcse two ccli types, we decided to stiiclv such ccli association, the resuts bcing reported here together with data un the Ircquency of thvir odurrence in ('('II cultures of human leukocytes of lx'althy subjccts, at different cultureles.Total ieukocytes were obtained from blood of healthy human donors (9 wornen, 8 unen; mean age 34, rangc 20-62). Venous blood was drained into svringes prcviouslv treated with phenul-Irce hcparin The piasma containing the whitc celis were seedeci in TC 199 medium plus penicilin-strepto- mycin. Samples of cultures were prepared at 48 and 96 hours by slight shaking of the respective flask. The celis were centrifugated at 200 g for 10 minutes and the superlaatant was discarded. The ce'] peliet vas gently resuspended in clrops of TC 199 mediurn. Drops of this cytopre para tion were put Oil gias.s sUdes for 5 min in a humidified charnber, and then dessicated by centrifugation on a disk perpendicular to the axi of the centrifuge, as prcviously described 1 . The cytopreparations were stai- ucd with May Criinwald-Giemsa, and observed at a Iight microscope, at 400 x.Tu considering that the monocytes WC) e found singly and centrally located in the rosettes we have described, the quantification of the rosettes vas made as the percentage of monocytes forming rosettes, by studving 50 of such celis in each case. A rosette was considc'recl when threc oc more lymphocvtes suirroiinded a rnonocytc.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 1992
url https://revistas.unc.edu.ar/index.php/med/article/view/45045
work_keys_str_mv AT cabralhumberto spontaneousformationofrosettesbyautologoushumanmonocytemacrophagesandlymphocytesincellcultures
AT novakivon spontaneousformationofrosettesbyautologoushumanmonocytemacrophagesandlymphocytesincellcultures
first_indexed 2025-02-05T22:05:39Z
last_indexed 2025-02-05T22:05:39Z
_version_ 1823256754559909888

Ejemplares similares