Protocol for thin section preparation of non decalcified human bone tissue

Mostrar todas las versiones(4)

Making thin sections from fossil and archaeological materials is usually a highly time consuming and expensive task. Also, the creation of a laboratory with necessary characteristics for the development of established protocols is also expensive. This paper presents an alternative protocol for obtai...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Navarro, Tamara Giselle, Jannello, Marcos, Cerda, Ignacio A., Béguelin, Marien, Vázquez, Romina
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Filosofía y Humanidades. Museo de Antropología 2018
Materias:
protocol
thin section
human bone remains
Histology
protocolo
corte delgado
restos óseos humanos
Histología
Acceso en línea:https://revistas.unc.edu.ar/index.php/antropologia/article/view/17007
Aporte de:
Revista del Museo de Antropología de Universidad Nacional de Córdoba
id I10-R372-article-17007
record_format ojs
spelling I10-R372-article-170072024-11-04T18:47:41Z Protocol for thin section preparation of non decalcified human bone tissue Protocolo para la preparación de secciones delgadas sin descalcificar de tejido óseo humano Navarro, Tamara Giselle Jannello, Marcos Cerda, Ignacio A. Béguelin, Marien Vázquez, Romina protocol thin section human bone remains Histology protocolo corte delgado restos óseos humanos Histología Making thin sections from fossil and archaeological materials is usually a highly time consuming and expensive task. Also, the creation of a laboratory with necessary characteristics for the development of established protocols is also expensive. This paper presents an alternative protocol for obtaining thin sections of human bone samples from archaeological sites with the aim of applying it to microstructural analysis. The procedures for obtaining thin cuts that are detailed here are within the reach of newly formed laboratories. In the first place a femur, a tibia and a first rib n adult individual from a contemporary cemetery was measured and recorded with digital images. Fragments of 1 cm of bone were them extracted. Molds and casts of the extracted samples were prepared before thin section preparation. The obtained results were optimal for the observation of the microstructural bone features. With the appropriate modifications (set time, abrasive grading according to the hardness of the material, impregnation, etc.) this protocol could be useful for several kinds of samples (extant, archaeological and even fossil ones). La obtención de cortes delgados de restos óseos correspondiente tanto a materiales fósiles como arqueológicos suele ser una tarea poco sencilla, lo cual conlleva generalmente un elevado costo y tiempos prolongados. Por otra parte, la formación de un laboratorio con las características necesarias para la realización de protocolos ya establecidos es sumamente costosa. En este trabajo se presenta un protocolo alternativo para la obtención de cortes delgados de muestras óseas humanas de sitios arqueológicos con el objetivo de aplicarlo al análisis microestructural. Los procedimientos de obtención de cortes delgados que aquí se detallan están al alcance de laboratorios de reciente formación. En primer lugar, se midió y se registró con imágenes digitales un fémur, una tibia y una primera costilla de un individuo adulto procedente de un cementerio contemporáneo. Luego se extrajeron fragmentos de 1 cm de hueso. Con el material se confeccionaron réplicas y por último se obtuvieron los cortes delgados. Los resultados obtenidos fueron óptimos para la observación de las características microestructurales del hueso. Con los ajustes adecuados (tiempo de fraguado, granulometría del abrasivo de acuerdo a la dureza del material, impregnación, etc.) este protocolo podría ser utilizado para diversos tipos de muestras actuales, arqueológicas e incluso fósiles. Facultad de Filosofía y Humanidades. Museo de Antropología 2018-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer reviewed article Artículo revisado por pares Investigación aplicada;Histología. Artigo revisado por pares application/pdf https://revistas.unc.edu.ar/index.php/antropologia/article/view/17007 10.31048/1852.4826.v11.n1.17007 Revista del Museo de Antropología; Vol. 11 No. 1 (2018); 121-126 Revista del Museo de Antropología; Vol. 11 Núm. 1 (2018); 121-126 Revista del Museo de Antropología; v. 11 n. 1 (2018); 121-126 1852-4826 1852-060X 10.31048/1852.4826.v11.n1 spa https://revistas.unc.edu.ar/index.php/antropologia/article/view/17007/20153 Derechos de autor 2018 Tamara Giselle Navarro; Marcos Jannello; Ignacio A. Cerda; Marien Béguelin; Romina Vázquez
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-372
container_title_str Revista del Museo de Antropología
language Español
format Artículo revista
topic protocol
thin section
human bone remains
Histology
protocolo
corte delgado
restos óseos humanos
Histología
spellingShingle protocol
thin section
human bone remains
Histology
protocolo
corte delgado
restos óseos humanos
Histología
Navarro, Tamara Giselle
Jannello, Marcos
Cerda, Ignacio A.
Béguelin, Marien
Vázquez, Romina
Protocol for thin section preparation of non decalcified human bone tissue
topic_facet protocol
thin section
human bone remains
Histology
protocolo
corte delgado
restos óseos humanos
Histología
author Navarro, Tamara Giselle
Jannello, Marcos
Cerda, Ignacio A.
Béguelin, Marien
Vázquez, Romina
author_facet Navarro, Tamara Giselle
Jannello, Marcos
Cerda, Ignacio A.
Béguelin, Marien
Vázquez, Romina
author_sort Navarro, Tamara Giselle
title Protocol for thin section preparation of non decalcified human bone tissue
title_short Protocol for thin section preparation of non decalcified human bone tissue
title_full Protocol for thin section preparation of non decalcified human bone tissue
title_fullStr Protocol for thin section preparation of non decalcified human bone tissue
title_full_unstemmed Protocol for thin section preparation of non decalcified human bone tissue
title_sort protocol for thin section preparation of non decalcified human bone tissue
description Making thin sections from fossil and archaeological materials is usually a highly time consuming and expensive task. Also, the creation of a laboratory with necessary characteristics for the development of established protocols is also expensive. This paper presents an alternative protocol for obtaining thin sections of human bone samples from archaeological sites with the aim of applying it to microstructural analysis. The procedures for obtaining thin cuts that are detailed here are within the reach of newly formed laboratories. In the first place a femur, a tibia and a first rib n adult individual from a contemporary cemetery was measured and recorded with digital images. Fragments of 1 cm of bone were them extracted. Molds and casts of the extracted samples were prepared before thin section preparation. The obtained results were optimal for the observation of the microstructural bone features. With the appropriate modifications (set time, abrasive grading according to the hardness of the material, impregnation, etc.) this protocol could be useful for several kinds of samples (extant, archaeological and even fossil ones).
publisher Facultad de Filosofía y Humanidades. Museo de Antropología
publishDate 2018
url https://revistas.unc.edu.ar/index.php/antropologia/article/view/17007
work_keys_str_mv AT navarrotamaragiselle protocolforthinsectionpreparationofnondecalcifiedhumanbonetissue
AT jannellomarcos protocolforthinsectionpreparationofnondecalcifiedhumanbonetissue
AT cerdaignacioa protocolforthinsectionpreparationofnondecalcifiedhumanbonetissue
AT beguelinmarien protocolforthinsectionpreparationofnondecalcifiedhumanbonetissue
AT vazquezromina protocolforthinsectionpreparationofnondecalcifiedhumanbonetissue
AT navarrotamaragiselle protocoloparalapreparaciondeseccionesdelgadassindescalcificardetejidooseohumano
AT jannellomarcos protocoloparalapreparaciondeseccionesdelgadassindescalcificardetejidooseohumano
AT cerdaignacioa protocoloparalapreparaciondeseccionesdelgadassindescalcificardetejidooseohumano
AT beguelinmarien protocoloparalapreparaciondeseccionesdelgadassindescalcificardetejidooseohumano
AT vazquezromina protocoloparalapreparaciondeseccionesdelgadassindescalcificardetejidooseohumano
first_indexed 2024-09-03T20:01:16Z
last_indexed 2025-03-27T05:32:41Z
_version_ 1827724131053862912

Ejemplares similares