Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner
Hyaluronan is a glycosaminoglycan normally present in the extracellular matrix in most tissues. Hyaluronan is a crucial player in many processes associated with cancer, such as angiogenesis, invasion, and metastasis. However, little has been reported regarding the action of hyaluronan on monocyte...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Otros Autores: | |
| Formato: | Artículo publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
2023
|
| Materias: | |
| Acceso en línea: | http://repositorio.unnoba.edu.ar/xmlui/handle/23601/632 |
| Aporte de: |
| id |
I103-R405-23601-632 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional del Noroeste de la Provincia de Buenos Aires |
| institution_str |
I-103 |
| repository_str |
R-405 |
| collection |
Re DI Repositorio Digital UNNOBA |
| language |
Inglés |
| topic |
Angiogenesis Breast carcinoma Colorectal carcinoma Hyaluronan Monocytes/macrophages TSG-6 |
| spellingShingle |
Angiogenesis Breast carcinoma Colorectal carcinoma Hyaluronan Monocytes/macrophages TSG-6 Spinelli, Fiorella Mercedes Vitale, Daiana Luján Icardi, Antonella Caon, Ilaria Brandone, Alejandra Giannoni, Paula Saturno, Virginia Passi, Alberto García, Mariana Sevic, Ina Alaniz, Laura Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner |
| topic_facet |
Angiogenesis Breast carcinoma Colorectal carcinoma Hyaluronan Monocytes/macrophages TSG-6 |
| description |
Hyaluronan is a glycosaminoglycan normally present in the extracellular
matrix in most tissues. Hyaluronan is a crucial player in many processes
associated with cancer, such as angiogenesis, invasion, and metastasis. However,
little has been reported regarding the action of hyaluronan on monocytes/
macrophages (Mo/MØ) in tumor angiogenesis and its consequences on
tumor development. In the present study, we investigated the effects of
hyaluronan of different sizes on human Mo/MØ angiogenic behavior in colorectal
and breast carcinoma. In vitro, the treatment of Mo/MØ with lysates
and conditioned media from a breast but not from colorectal carcinoma cell
line plus high-molecular weight hyaluronan induced: (a) an increased expression
of angiogenic factors VEGF, IL-8, FGF-2, and MMP-2, (b) an
increased endothelial cell migration, and (c) a differential expression of
hyaluronan-binding protein TSG-6. Similar results were observed in Mo/
MØ derived from breast cancer patients treated with tumor lysates. Besides,
macrophages primed with high-molecular weight hyaluronan and inoculated
in human breast cancer xenograft tumor increased blood vessel formation
and diminished TSG-6 levels. In contrast, the effects triggered by highmolecular
weight hyaluronan on Mo/MØ in breast cancer context were not
observed in the context of colorectal carcinoma. Taken together, these results
indicate that the effect of high-molecular weight hyaluronan as an inductor
of the angiogenic behavior of macrophages in breast tumor context is in part
consequence of the presence of TSG-6. |
| author2 |
0000-0001-5593-9101 |
| author_facet |
0000-0001-5593-9101 Spinelli, Fiorella Mercedes Vitale, Daiana Luján Icardi, Antonella Caon, Ilaria Brandone, Alejandra Giannoni, Paula Saturno, Virginia Passi, Alberto García, Mariana Sevic, Ina Alaniz, Laura |
| format |
Artículo Artículo publishedVersion Artículo Artículo publishedVersion Artículo Artículo publishedVersion |
| author |
Spinelli, Fiorella Mercedes Vitale, Daiana Luján Icardi, Antonella Caon, Ilaria Brandone, Alejandra Giannoni, Paula Saturno, Virginia Passi, Alberto García, Mariana Sevic, Ina Alaniz, Laura |
| author_sort |
Spinelli, Fiorella Mercedes |
| title |
Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner |
| title_short |
Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner |
| title_full |
Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner |
| title_fullStr |
Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner |
| title_full_unstemmed |
Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner |
| title_sort |
hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of tsg-6 expression in a tumor type-specific manner |
| publishDate |
2023 |
| url |
http://repositorio.unnoba.edu.ar/xmlui/handle/23601/632 |
| work_keys_str_mv |
AT spinellifiorellamercedes hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT vitaledaianalujan hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT icardiantonella hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT caonilaria hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT brandonealejandra hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT giannonipaula hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT saturnovirginia hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT passialberto hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT garciamariana hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT sevicina hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner AT alanizlaura hyaluronanpreconditioningofmonocytesmacrophagesaffectstheirangiogenicbehaviorandregulationoftsg6expressioninatumortypespecificmanner |
| _version_ |
1850060745067200512 |
| spelling |
I103-R405-23601-6322025-10-21T21:09:11Z Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner Spinelli, Fiorella Mercedes Vitale, Daiana Luján Icardi, Antonella Caon, Ilaria Brandone, Alejandra Giannoni, Paula Saturno, Virginia Passi, Alberto García, Mariana Sevic, Ina Alaniz, Laura 0000-0001-5593-9101 0000-0003-3328-3089 0000-0002-6070-6078 Angiogenesis Breast carcinoma Colorectal carcinoma Hyaluronan Monocytes/macrophages TSG-6 Hyaluronan is a glycosaminoglycan normally present in the extracellular matrix in most tissues. Hyaluronan is a crucial player in many processes associated with cancer, such as angiogenesis, invasion, and metastasis. However, little has been reported regarding the action of hyaluronan on monocytes/ macrophages (Mo/MØ) in tumor angiogenesis and its consequences on tumor development. In the present study, we investigated the effects of hyaluronan of different sizes on human Mo/MØ angiogenic behavior in colorectal and breast carcinoma. In vitro, the treatment of Mo/MØ with lysates and conditioned media from a breast but not from colorectal carcinoma cell line plus high-molecular weight hyaluronan induced: (a) an increased expression of angiogenic factors VEGF, IL-8, FGF-2, and MMP-2, (b) an increased endothelial cell migration, and (c) a differential expression of hyaluronan-binding protein TSG-6. Similar results were observed in Mo/ MØ derived from breast cancer patients treated with tumor lysates. Besides, macrophages primed with high-molecular weight hyaluronan and inoculated in human breast cancer xenograft tumor increased blood vessel formation and diminished TSG-6 levels. In contrast, the effects triggered by highmolecular weight hyaluronan on Mo/MØ in breast cancer context were not observed in the context of colorectal carcinoma. Taken together, these results indicate that the effect of high-molecular weight hyaluronan as an inductor of the angiogenic behavior of macrophages in breast tumor context is in part consequence of the presence of TSG-6. Fil: Spinelli, Fiorella Mercedes. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Spinelli, Fiorella Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Fil: Vitale, Daiana Luján. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Vitale, Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Fil: Icardi, Antonella. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Icardi, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Fil: Caon, Ilaria. Università degli Studio dell’Insubria. Dipartimento di Medicina e Chirurgia; Italia. Fil: Brandone, Alejandra. Hospital Interzonal General de Agudos Dr. Abraham F. Piñeyro; Argentina. Fil: Giannoni, Paula. Clínica Centro; Argentina. Fil: Saturno, Virginia. Hospital Interzonal General de Agudos Dr. Abraham F. Piñeyro; Argentina. Fil: Passi, Alberto. Università degli Studio dell’Insubria. Dipartimento di Medicina e Chirurgia; Italia. Fil: García, Mariana. Universidad Austral. IIMT–CONICET. Laboratorio de Terapia Génica; Argentina. Fil: Sevic, Ina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Sevic, Ina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Fil: Alaniz, Laura. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones Básicas y Aplicadas. Laboratorio de Microambiente Tumoral; Argentina. Fil: Alaniz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentina. Con referato 2023-12-04T18:38:40Z 2023-12-04T18:38:40Z 2019-04-29 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion Spinelli F.M., Vitale D.L., Icardi A., Caon I., Brandone A., Giannoni P., Saturno V., Passi A., García M., Sevic I., Alaniz L. (2019). Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG-6 expression in a tumor type-specific manner. The FEBS Journal, 286(17), 3433-3449. 1742-4658 http://repositorio.unnoba.edu.ar/xmlui/handle/23601/632 eng eu-repo/grantAgreement/Ministerio de Salud/Instituto Nacional del Cáncer/PIO 2015/ID31-2015 eu-repo/grantAgreement/European Comission/H2020 RISE-MSCA Project/645,756/GLYCANC eu-repo/grantAgreement/UNNOBA/SIB 2017/01117-2017 eu-repo/grantAgreement/Fundación Roemmers 2018 doi:10.1111/febs.14871 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf application/pdf The FEBS Journal |