Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway

Beneficial bacteria have been shown to affect host longevity, but the molecular mechanisms mediating such effects remain largely unclear. Here we show that formation of Bacillus subtilis biofilms increases Caenorhabditis elegans lifespan. Biofilm-proficient B. subtilis colonizes the C. elegans gu...

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Detalles Bibliográficos
Autores principales: Donato, Verónica, Rodríguez Ayala, Facundo, Cogliati, Sebastián, Bauman, Carlos, Costa, Juan Gabriel, Leñini, Cecilia, Grau, Roberto Ricardo
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Nature 2021
Materias:
Bacillus subtilis
Biofilms
Caenorhabditis
Longevity
Acceso en línea:http://hdl.handle.net/2133/20159
http://hdl.handle.net/2133/20159
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20159
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Bacillus subtilis
Biofilms
Caenorhabditis
Longevity
spellingShingle Bacillus subtilis
Biofilms
Caenorhabditis
Longevity
Donato, Verónica
Rodríguez Ayala, Facundo
Cogliati, Sebastián
Bauman, Carlos
Costa, Juan Gabriel
Leñini, Cecilia
Grau, Roberto Ricardo
Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway
topic_facet Bacillus subtilis
Biofilms
Caenorhabditis
Longevity
description Beneficial bacteria have been shown to affect host longevity, but the molecular mechanisms mediating such effects remain largely unclear. Here we show that formation of Bacillus subtilis biofilms increases Caenorhabditis elegans lifespan. Biofilm-proficient B. subtilis colonizes the C. elegans gut and extends worm lifespan more than biofilm-deficient isogenic strains. Two molecules produced by B. subtilis — the quorum-sensing pentapeptide CSF and nitric oxide (NO) — are sufficient to extend C. elegans longevity. When B. subtilis is cultured under biofilm-supporting conditions, the synthesis of NO and CSF is increased in comparison with their production under planktonic growth conditions. We further show that the prolongevity effect of B. subtilis biofilms depends on the DAF-2/DAF-16/HSF-1 signalling axis and the downregulation of the insulin-like signalling (ILS) pathway.
format publishedVersion
author Donato, Verónica
Rodríguez Ayala, Facundo
Cogliati, Sebastián
Bauman, Carlos
Costa, Juan Gabriel
Leñini, Cecilia
Grau, Roberto Ricardo
author_facet Donato, Verónica
Rodríguez Ayala, Facundo
Cogliati, Sebastián
Bauman, Carlos
Costa, Juan Gabriel
Leñini, Cecilia
Grau, Roberto Ricardo
author_sort Donato, Verónica
title Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway
title_short Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway
title_full Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway
title_fullStr Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway
title_full_unstemmed Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway
title_sort bacillus subtilis biofilm extends caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway
publisher Nature
publishDate 2021
url http://hdl.handle.net/2133/20159
http://hdl.handle.net/2133/20159
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