Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells

Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning elec...

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Autores principales:	Cacicedo, Maximiliano Luis, León, Ignacio Esteban, Gonzalez, Jimena Soledad, Porto, Luismar M., Alvarez, Vera Alejandra, Castro, Guillermo Raúl
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2016
Materias:	Ciencias Exactas Bacterial cellulose Alginate Drug delivery Nanocomposite Doxorubicin Cancer therapy Human colorectal ht-29 cells
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/100753 https://ri.conicet.gov.ar/11336/34537
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-100753
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Bacterial cellulose Alginate Drug delivery Nanocomposite Doxorubicin Cancer therapy Human colorectal ht-29 cells
spellingShingle	Ciencias Exactas Bacterial cellulose Alginate Drug delivery Nanocomposite Doxorubicin Cancer therapy Human colorectal ht-29 cells Cacicedo, Maximiliano Luis León, Ignacio Esteban Gonzalez, Jimena Soledad Porto, Luismar M. Alvarez, Vera Alejandra Castro, Guillermo Raúl Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
topic_facet	Ciencias Exactas Bacterial cellulose Alginate Drug delivery Nanocomposite Doxorubicin Cancer therapy Human colorectal ht-29 cells
description	Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer?Emmett?Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett?Joyner?Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film.Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95?53% after 24 h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.
format	Articulo Preprint
author	Cacicedo, Maximiliano Luis León, Ignacio Esteban Gonzalez, Jimena Soledad Porto, Luismar M. Alvarez, Vera Alejandra Castro, Guillermo Raúl
author_facet	Cacicedo, Maximiliano Luis León, Ignacio Esteban Gonzalez, Jimena Soledad Porto, Luismar M. Alvarez, Vera Alejandra Castro, Guillermo Raúl
author_sort	Cacicedo, Maximiliano Luis
title	Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_short	Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_full	Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_fullStr	Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_full_unstemmed	Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_sort	modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal ht-29 cells
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/100753 https://ri.conicet.gov.ar/11336/34537
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bdutipo_str	Repositorios
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Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells

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