Development and in-house validation of a real-time polymerase chain reaction for the detection of <i>Listeria monocytogenes</i> in meat

Listeriosis is a foodborne disease caused by Listeria monocytogenes. The aims of this work were to develop and validate an in-house real-time polymerase chain reaction (RT-PCR) for the detection of L. monocytogenes, and to determine its prevalence in raw ground beef samples from 53 butcheries that a...

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Autores principales: Reyes Álvarez, Camilo Andrés, Linares, Luciano Héctor, Moredo, Fabiana Alicia, Lirón, Juan Pedro, Brusa, Victoria, Londero, Alejandra, Galli, Lucía, Oteiza, Juan Martín, Costa, Magdalena, Leotta, Gerardo Aníbal
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Biología
Listeria
Detection
RT-PCR
Validation
Meat
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103744
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103744
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biología
Listeria
Detection
RT-PCR
Validation
Meat
spellingShingle Química
Biología
Listeria
Detection
RT-PCR
Validation
Meat
Reyes Álvarez, Camilo Andrés
Linares, Luciano Héctor
Moredo, Fabiana Alicia
Lirón, Juan Pedro
Brusa, Victoria
Londero, Alejandra
Galli, Lucía
Oteiza, Juan Martín
Costa, Magdalena
Leotta, Gerardo Aníbal
Development and in-house validation of a real-time polymerase chain reaction for the detection of <i>Listeria monocytogenes</i> in meat
topic_facet Química
Biología
Listeria
Detection
RT-PCR
Validation
Meat
description Listeriosis is a foodborne disease caused by Listeria monocytogenes. The aims of this work were to develop and validate an in-house real-time polymerase chain reaction (RT-PCR) for the detection of L. monocytogenes, and to determine its prevalence in raw ground beef samples from 53 butcheries that also sell ready-to-eat foods. One set of primers and one hydrolysis probe were designed for hly gene detection and then challenged with pure strains. The detection was successful for all L. monocytogenes strains analyzed and negative for all non-L. monocytogenes strains (detection limit, 10 colony forming unit [CFU]/mL). Inclusivity, exclusivity, and analytical accuracy were 100%. L. monocytogenes was detected in 41.5% of raw ground beef samples from the 53 butcheries analyzed. This RT-PCR may be a valuable method for rapid detection of L. monocytogenes in meat.
format Articulo
Preprint
author Reyes Álvarez, Camilo Andrés
Linares, Luciano Héctor
Moredo, Fabiana Alicia
Lirón, Juan Pedro
Brusa, Victoria
Londero, Alejandra
Galli, Lucía
Oteiza, Juan Martín
Costa, Magdalena
Leotta, Gerardo Aníbal
author_facet Reyes Álvarez, Camilo Andrés
Linares, Luciano Héctor
Moredo, Fabiana Alicia
Lirón, Juan Pedro
Brusa, Victoria
Londero, Alejandra
Galli, Lucía
Oteiza, Juan Martín
Costa, Magdalena
Leotta, Gerardo Aníbal
author_sort Reyes Álvarez, Camilo Andrés
title Development and in-house validation of a real-time polymerase chain reaction for the detection of <i>Listeria monocytogenes</i> in meat
title_short Development and in-house validation of a real-time polymerase chain reaction for the detection of <i>Listeria monocytogenes</i> in meat
title_full Development and in-house validation of a real-time polymerase chain reaction for the detection of <i>Listeria monocytogenes</i> in meat
title_fullStr Development and in-house validation of a real-time polymerase chain reaction for the detection of <i>Listeria monocytogenes</i> in meat
title_full_unstemmed Development and in-house validation of a real-time polymerase chain reaction for the detection of <i>Listeria monocytogenes</i> in meat
title_sort development and in-house validation of a real-time polymerase chain reaction for the detection of <i>listeria monocytogenes</i> in meat
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103744
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