Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta

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The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Rega...

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Autores principales: Diessler, Mónica Elizabeth, Ventureira, M., Hernández, Rocío, Sobarzo, C., Casas, Luciano Agustín, Barbeito, Claudio Gustavo, Cebral, Elisa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
Matrix metalloproteinases
Dog placenta
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103751
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103751
record_format dspace
spelling I19-R120-10915-1037512024-10-03T18:57:55Z http://sedici.unlp.edu.ar/handle/10915/103751 Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta Diessler, Mónica Elizabeth Ventureira, M. Hernández, Rocío Sobarzo, C. Casas, Luciano Agustín Barbeito, Claudio Gustavo Cebral, Elisa 2017 2020-09-03T15:13:58Z en Ciencias Veterinarias Matrix metalloproteinases Dog placenta The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the expression of MMP enzymes has been studied in the cat and the mature canine placenta. The aim of this study was to analyse the expression and activity of MMP-2 and MMP-9 in the early dog placenta. Placentae from 18 to 30 days of pregnancy were collected from four bitches. Two placentae from each bitch were analysed. Placental tissue from one uterine horn was fixed in formaldehyde for immunohistochemistry, while marginal haematoma, labyrinth, non-implantative and implantative endometrium from the contralateral horn were immediately frozen in dry ice for the analysis of MMP expression (Western blot [WB]) and activity (zymography). MMP-2 and MMP-9 were evidenced in the labyrinth, maternal glands and marginal haematoma; this finding was directly correlated with levels of MMP expression by WB, and with the activity of MMP-2, mainly in the haematoma (the area of major remodelling of tissues). Thus, although MMP-9 is well expressed in the early canine placenta, it is not active. Given the important role of MMPs for invasiveness, maternal–foetal angiogenesis and the establishment of a correct foetal nutrition, the results are consistent with the findings in other species in which the MMP-2 activation precedes the MMP-9 one in early placentation. Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Matrix metalloproteinases
Dog placenta
spellingShingle Ciencias Veterinarias
Matrix metalloproteinases
Dog placenta
Diessler, Mónica Elizabeth
Ventureira, M.
Hernández, Rocío
Sobarzo, C.
Casas, Luciano Agustín
Barbeito, Claudio Gustavo
Cebral, Elisa
Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
topic_facet Ciencias Veterinarias
Matrix metalloproteinases
Dog placenta
description The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (MMP) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the expression of MMP enzymes has been studied in the cat and the mature canine placenta. The aim of this study was to analyse the expression and activity of MMP-2 and MMP-9 in the early dog placenta. Placentae from 18 to 30 days of pregnancy were collected from four bitches. Two placentae from each bitch were analysed. Placental tissue from one uterine horn was fixed in formaldehyde for immunohistochemistry, while marginal haematoma, labyrinth, non-implantative and implantative endometrium from the contralateral horn were immediately frozen in dry ice for the analysis of MMP expression (Western blot [WB]) and activity (zymography). MMP-2 and MMP-9 were evidenced in the labyrinth, maternal glands and marginal haematoma; this finding was directly correlated with levels of MMP expression by WB, and with the activity of MMP-2, mainly in the haematoma (the area of major remodelling of tissues). Thus, although MMP-9 is well expressed in the early canine placenta, it is not active. Given the important role of MMPs for invasiveness, maternal–foetal angiogenesis and the establishment of a correct foetal nutrition, the results are consistent with the findings in other species in which the MMP-2 activation precedes the MMP-9 one in early placentation.
format Articulo
Articulo
author Diessler, Mónica Elizabeth
Ventureira, M.
Hernández, Rocío
Sobarzo, C.
Casas, Luciano Agustín
Barbeito, Claudio Gustavo
Cebral, Elisa
author_facet Diessler, Mónica Elizabeth
Ventureira, M.
Hernández, Rocío
Sobarzo, C.
Casas, Luciano Agustín
Barbeito, Claudio Gustavo
Cebral, Elisa
author_sort Diessler, Mónica Elizabeth
title Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_short Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_full Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_fullStr Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_full_unstemmed Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
title_sort differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103751
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