A rapid and simple method for the determination of psychoactive alkaloids by CE-UV: application to Peganum Harmala seed infusions

The β-carboline alkaloids of the harmala (HAlks) group are compounds widely spread in many natural sources, but found at relatively high levels in some specific plants like Peganum harmala (Syrian rue) or Banisteriopsis caapi. HAlks are a reversible Mono Amino Oxidase type A Inhibitor (MAOI) and, as...

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Autores principales: Tascon, Marcos, Benavente, Fernando, Vizioli, Nora M., Gagliardi, Leonardo Gabriel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Capillary electrophoresis
β-carboline
Harmala alkaloids
Optimization
Peganum harmala
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103780
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103780
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Capillary electrophoresis
β-carboline
Harmala alkaloids
Optimization
Peganum harmala
spellingShingle Química
Capillary electrophoresis
β-carboline
Harmala alkaloids
Optimization
Peganum harmala
Tascon, Marcos
Benavente, Fernando
Vizioli, Nora M.
Gagliardi, Leonardo Gabriel
A rapid and simple method for the determination of psychoactive alkaloids by CE-UV: application to Peganum Harmala seed infusions
topic_facet Química
Capillary electrophoresis
β-carboline
Harmala alkaloids
Optimization
Peganum harmala
description The β-carboline alkaloids of the harmala (HAlks) group are compounds widely spread in many natural sources, but found at relatively high levels in some specific plants like Peganum harmala (Syrian rue) or Banisteriopsis caapi. HAlks are a reversible Mono Amino Oxidase type A Inhibitor (MAOI) and, as a consequence, these plants or their extracts can be used to produce psychotropic effects when are combined with psychotropic drugs based on amino groups. Since the occurrence and the levels of the HAlks in natural sources are subject to significant variability, more widespread use is not clinical but recreational or ritual, for example B. caapi is a known part of the Ayahuasca ritual mixture. The lack of simple methods to control the variable levels of these compounds in natural sources restricts the possibilities to dose in strict quantities and, as a consequence, limits its use with pharmacological or clinical purposes. In this work, we present a fast, simple, and robust method of quantifying simultaneously the six HAlksmore frequently found in plants, i.e., harmine, harmaline, harmol, harmalol, harmane, and norharmane, by capillary electrophoresis instruments equipped with the more common detector UV. The method is applied to analyze these HAlks in P. Harmala seeds infusion which is a frequent intake form for these HAlks. The method is validated in three different instruments in order to evaluate the transferability and to compare the performances between them. In this case, harmaline, harmine, and harmol were found in the infusion samples.
format Articulo
Articulo
author Tascon, Marcos
Benavente, Fernando
Vizioli, Nora M.
Gagliardi, Leonardo Gabriel
author_facet Tascon, Marcos
Benavente, Fernando
Vizioli, Nora M.
Gagliardi, Leonardo Gabriel
author_sort Tascon, Marcos
title A rapid and simple method for the determination of psychoactive alkaloids by CE-UV: application to Peganum Harmala seed infusions
title_short A rapid and simple method for the determination of psychoactive alkaloids by CE-UV: application to Peganum Harmala seed infusions
title_full A rapid and simple method for the determination of psychoactive alkaloids by CE-UV: application to Peganum Harmala seed infusions
title_fullStr A rapid and simple method for the determination of psychoactive alkaloids by CE-UV: application to Peganum Harmala seed infusions
title_full_unstemmed A rapid and simple method for the determination of psychoactive alkaloids by CE-UV: application to Peganum Harmala seed infusions
title_sort rapid and simple method for the determination of psychoactive alkaloids by ce-uv: application to peganum harmala seed infusions
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103780
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