Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn

After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected...

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Autores principales: Buthet, Lara Romina, Maciel, María Eugenia, Quintans, Leandro, Rodríguez De Castro, Carmen, Costantini, Martin Hernán, Fanelli, Silvia Laura, Castro, Jose Alberto, Castro, Gerardo Daniel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104493
http://hdl.handle.net/11336/76405
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104493
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
spellingShingle Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
Buthet, Lara Romina
Maciel, María Eugenia
Quintans, Leandro
Rodríguez De Castro, Carmen
Costantini, Martin Hernán
Fanelli, Silvia Laura
Castro, Jose Alberto
Castro, Gerardo Daniel
Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
topic_facet Biología
Uterus
Ethanol
Acetaldehyde
Oxidative stress
Reproductive toxicity
Alcohol
description After alcohol exposure through a standard Lieber and De Carli diet for 28 days, a severe atrophy in the rat uteirne horn was observed, accompanied by significant alterations in its epithelial cells. Microsomal pathway of acetaldehyde production was slightly increased. Hydroxyl radicals were detected in the cytosolic fraction, and this was attributed to participation of xanthine oxidoreductase. They were also observed in the microsomal fraction in the presence of NADPH generating system. No generation of 1-hydroxyethyl was evidenced. The t-butylhydroperoxide-induced chemiluminescence analysis of uterine horn homogenates revealed a significant increase in the chemiluminiscence emission due to ethanol exposure. In the animals repeatedly exposed to alcohol, sulfhydryl content from uterine horn proteins was decreased, but no significant changes were observed in the protein carbonyl content from the same samples. Minor but significant decreasing changes were observed in the GSH content accompanied by a tendency to decrease in the GSH/GSSG ratio. A highly significant finding was the diminished activity content of glutathione peroxidase. Results suggest that acetaldehyde accumulation plus the oxidative stress may play an additional effect to the alcohol-promoted hormonal changes in the uterus reported by others after chronic exposure to alcohol.
format Articulo
Articulo
author Buthet, Lara Romina
Maciel, María Eugenia
Quintans, Leandro
Rodríguez De Castro, Carmen
Costantini, Martin Hernán
Fanelli, Silvia Laura
Castro, Jose Alberto
Castro, Gerardo Daniel
author_facet Buthet, Lara Romina
Maciel, María Eugenia
Quintans, Leandro
Rodríguez De Castro, Carmen
Costantini, Martin Hernán
Fanelli, Silvia Laura
Castro, Jose Alberto
Castro, Gerardo Daniel
author_sort Buthet, Lara Romina
title Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_short Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_full Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_fullStr Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_full_unstemmed Acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
title_sort acetaldehyde content and oxidative stress in the deleterious effects of alcohol drinking on rat uterine horn
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/104493
http://hdl.handle.net/11336/76405
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