Junín virus induces autophagy in human A549 cells

Autophagy, a highly regulated degradative process that promotes cellular homeostasis, is increasingly recognised as a fundamental component of the cellular response against viral infection. In this study, we investigated the role of autophagy during Junı´n virus (JUNV) multiplication using human A54...

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Autores principales: Pérez Vidakovics, María Laura Anabella, Ure, Agustín Enrique, Arrías, Paula Nazarena, Romanowski, Víctor, Gómez, Ricardo Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Biología
Autophagy
Junín virus
A549 cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107790
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6583977&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107790
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Autophagy
Junín virus
A549 cells
spellingShingle Biología
Autophagy
Junín virus
A549 cells
Pérez Vidakovics, María Laura Anabella
Ure, Agustín Enrique
Arrías, Paula Nazarena
Romanowski, Víctor
Gómez, Ricardo Martín
Junín virus induces autophagy in human A549 cells
topic_facet Biología
Autophagy
Junín virus
A549 cells
description Autophagy, a highly regulated degradative process that promotes cellular homeostasis, is increasingly recognised as a fundamental component of the cellular response against viral infection. In this study, we investigated the role of autophagy during Junı´n virus (JUNV) multiplication using human A549 cells. We found that JUNV infection induces an increment of the LC3-II/LC3-I ratio, an accumulation of punctate pattern in RFP-LC3-transfected cells and the colocalisation of viral nucleoprotein and LC3 protein, suggesting autophagosome formation. JUNV infection also induced the degradation of the autophagy receptor p62, suggesting that complete autophagic flux was triggered. In addition, we showed that inhibition of autophagy with bafilomycin A1 or 3-methyladenine significantly reduces viral multiplication. Moreover, viral yield was increased when autophagy was induced using rapamycin. Furthermore, JUNV infection induced the colocalisation of p62, ATG16, RAB5, RAB7A and LAMP1 with the autophagosomal LC3 protein. That suggests that phagosomes undergo the maturation process during viral infection. Finally, we demonstrated that siRNA experiments targeting essential autophagy genes (ATG5, ATG7 and Beclin 1) reduce viral protein synthesis and viral yield. Overall, our results indicate that JUNV activates host autophagy machinery enhancing its multiplication.
format Articulo
Articulo
author Pérez Vidakovics, María Laura Anabella
Ure, Agustín Enrique
Arrías, Paula Nazarena
Romanowski, Víctor
Gómez, Ricardo Martín
author_facet Pérez Vidakovics, María Laura Anabella
Ure, Agustín Enrique
Arrías, Paula Nazarena
Romanowski, Víctor
Gómez, Ricardo Martín
author_sort Pérez Vidakovics, María Laura Anabella
title Junín virus induces autophagy in human A549 cells
title_short Junín virus induces autophagy in human A549 cells
title_full Junín virus induces autophagy in human A549 cells
title_fullStr Junín virus induces autophagy in human A549 cells
title_full_unstemmed Junín virus induces autophagy in human A549 cells
title_sort junín virus induces autophagy in human a549 cells
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/107790
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6583977&blobtype=pdf
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bdutipo_str Repositorios
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