Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties

Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from Moringa oleifera...

Descripción completa

Detalles Bibliográficos
Autores principales: Cotabarren, Juliana, Claver, Santiago Pablo, Abreu Payrol, Juan, García Pardo, Javier, Obregón, Walter David
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Bioquímica
Ciencias Exactas
Moringa oleifera
protease
plant protease inhibitor
phytocystatin
papain inhibitor
antibacterial
anticoagulant
bioactive compound
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/123821
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-123821
record_format dspace
spelling I19-R120-10915-1238212023-10-09T15:03:38Z http://sedici.unlp.edu.ar/handle/10915/123821 Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties Cotabarren, Juliana Claver, Santiago Pablo Abreu Payrol, Juan García Pardo, Javier Obregón, Walter David 2021-04-08 2021-08-31T14:57:16Z en Bioquímica Ciencias Exactas Moringa oleifera protease plant protease inhibitor phytocystatin papain inhibitor antibacterial anticoagulant bioactive compound Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from Moringa oleifera (MoPI). MoPI has a molecular mass of 19 kDa and showed an extraordinary physicochemical stability against acidic pHs and high temperatures. Our findings also revealed that MoPI is one of the most potent cysteine protease inhibitors reported to date, with Ki and IC50 values of 2.1 nM and 5.7 nM, respectively. More interestingly, MoPI presents a strong antimicrobial activity against human pathogens such as Enterococcus faecalis and Staphylococcus aureus. In addition, MoPI also showed important anticoagulant activity, which is an unprecedented property for this family of protease inhibitors. These results highlight the pharmaceutical potential of this plant and its derived bioactive molecules. Centro de Investigación de Proteínas Vegetales Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Ciencias Exactas
Moringa oleifera
protease
plant protease inhibitor
phytocystatin
papain inhibitor
antibacterial
anticoagulant
bioactive compound
spellingShingle Bioquímica
Ciencias Exactas
Moringa oleifera
protease
plant protease inhibitor
phytocystatin
papain inhibitor
antibacterial
anticoagulant
bioactive compound
Cotabarren, Juliana
Claver, Santiago Pablo
Abreu Payrol, Juan
García Pardo, Javier
Obregón, Walter David
Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties
topic_facet Bioquímica
Ciencias Exactas
Moringa oleifera
protease
plant protease inhibitor
phytocystatin
papain inhibitor
antibacterial
anticoagulant
bioactive compound
description Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from Moringa oleifera (MoPI). MoPI has a molecular mass of 19 kDa and showed an extraordinary physicochemical stability against acidic pHs and high temperatures. Our findings also revealed that MoPI is one of the most potent cysteine protease inhibitors reported to date, with Ki and IC50 values of 2.1 nM and 5.7 nM, respectively. More interestingly, MoPI presents a strong antimicrobial activity against human pathogens such as Enterococcus faecalis and Staphylococcus aureus. In addition, MoPI also showed important anticoagulant activity, which is an unprecedented property for this family of protease inhibitors. These results highlight the pharmaceutical potential of this plant and its derived bioactive molecules.
format Articulo
Articulo
author Cotabarren, Juliana
Claver, Santiago Pablo
Abreu Payrol, Juan
García Pardo, Javier
Obregón, Walter David
author_facet Cotabarren, Juliana
Claver, Santiago Pablo
Abreu Payrol, Juan
García Pardo, Javier
Obregón, Walter David
author_sort Cotabarren, Juliana
title Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties
title_short Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties
title_full Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties
title_fullStr Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties
title_full_unstemmed Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties
title_sort purification and characterization of a novel thermostable papain inhibitor from moringa oleifera with antimicrobial and anticoagulant properties
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/123821
work_keys_str_mv AT cotabarrenjuliana purificationandcharacterizationofanovelthermostablepapaininhibitorfrommoringaoleiferawithantimicrobialandanticoagulantproperties
AT claversantiagopablo purificationandcharacterizationofanovelthermostablepapaininhibitorfrommoringaoleiferawithantimicrobialandanticoagulantproperties
AT abreupayroljuan purificationandcharacterizationofanovelthermostablepapaininhibitorfrommoringaoleiferawithantimicrobialandanticoagulantproperties
AT garciapardojavier purificationandcharacterizationofanovelthermostablepapaininhibitorfrommoringaoleiferawithantimicrobialandanticoagulantproperties
AT obregonwalterdavid purificationandcharacterizationofanovelthermostablepapaininhibitorfrommoringaoleiferawithantimicrobialandanticoagulantproperties
_version_ 1807220625669881856

Ejemplares similares