Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity

The role of TlyA, TlyB and TlyC proteins in the biology of <i>Leptospira</i> is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments...

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Detalles Bibliográficos
Autores principales: Carvalho, Eneas, Barbosa, Angela S., Gómez, Ricardo Martín, Cianciarullo, Aurora M., Hauk, Pricila, Abreu, Patrícia A., Fiorini, Leonardo C., Oliveira, Maria L. S., Romero, Eliete C., Gonçales, Amane P., Morais, Zenaide M., Vasconcellos, Sílvio A., Ho, Paulo L.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127120
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127120
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
spellingShingle Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Cianciarullo, Aurora M.
Hauk, Pricila
Abreu, Patrícia A.
Fiorini, Leonardo C.
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Sílvio A.
Ho, Paulo L.
Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
topic_facet Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
description The role of TlyA, TlyB and TlyC proteins in the biology of <i>Leptospira</i> is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed protein that seems to bind to laminin, collagen IV and fibronectin. The expression of both proteins was detected both in vitro and in vivo. Our findings suggest that TlyB and TlyC are not directly involved in hemolysis, and that TlyC may contribute to <i>Leptospira</i> binding to extracellular matrix (ECM) during host infection.
format Articulo
Articulo
author Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Cianciarullo, Aurora M.
Hauk, Pricila
Abreu, Patrícia A.
Fiorini, Leonardo C.
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Sílvio A.
Ho, Paulo L.
author_facet Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Cianciarullo, Aurora M.
Hauk, Pricila
Abreu, Patrícia A.
Fiorini, Leonardo C.
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Sílvio A.
Ho, Paulo L.
author_sort Carvalho, Eneas
title Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_short Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_full Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_fullStr Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_full_unstemmed Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_sort leptospiral tlyc is an extracellular matrix-binding protein and does not present hemolysin activity
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/127120
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