NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale

Single-particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano-resolved population dynamics of QDs is exploited to reconstruct the topography and s...

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Detalles Bibliográficos
Autores principales:	Tasso, Mariana, Pons, Thomas, Lequeux, Nicolas, Nguyen, Julie, Lenkei, Zsolt, Zala, Diana
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2019
Materias:	Física Biophysics Membrane protein Super-resolution microscopy Materials science Transmembrane protein Cell membrane Population Synaptic cleft Membrane Filopodia Cannabinoid receptor type 1 Neuronal plasticity Quantum dots Synapses
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/127302
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-127302
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Física Biophysics Membrane protein Super-resolution microscopy Materials science Transmembrane protein Cell membrane Population Synaptic cleft Membrane Filopodia Cannabinoid receptor type 1 Neuronal plasticity Quantum dots Synapses
spellingShingle	Física Biophysics Membrane protein Super-resolution microscopy Materials science Transmembrane protein Cell membrane Population Synaptic cleft Membrane Filopodia Cannabinoid receptor type 1 Neuronal plasticity Quantum dots Synapses Tasso, Mariana Pons, Thomas Lequeux, Nicolas Nguyen, Julie Lenkei, Zsolt Zala, Diana NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
topic_facet	Física Biophysics Membrane protein Super-resolution microscopy Materials science Transmembrane protein Cell membrane Population Synaptic cleft Membrane Filopodia Cannabinoid receptor type 1 Neuronal plasticity Quantum dots Synapses
description	Single-particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano-resolved population dynamics of QDs is exploited to reconstruct the topography and structural changes of the cell membrane surface with high temporal and spatial resolution. For this proof-of-concept study, bright, small, and stable biofunctional QD nanoconstructs are utilized recognizing the endogenous neuronal cannabinoid receptor 1, a highly expressed and fast-diffusing membrane protein, together with a commercial point-localization microscope. Rapid QD diffusion on the axonal plasma membrane of cultured hippocampal neurons allows precise reconstruction of the membrane surface in less than 1 min with a spatial resolution of tens of nanometers. Access of the QD nanoconstructs to the synaptic cleft enables rapid 3D topological reconstruction of the entire presynaptic component. Successful reconstruction of membrane nano-topology and deformation at the second time-scale is also demonstrated for HEK293 cell filopodia and axons. Named "nanoPaint," this super-resolution imaging technique amenable to any endogenous transmembrane target represents a versatile platform to rapidly and accurately reconstruct the cell membrane nano-topography, thereby enabling the study of the rapid dynamic phenomena involved in neuronal membrane plasticity.
format	Articulo Articulo
author	Tasso, Mariana Pons, Thomas Lequeux, Nicolas Nguyen, Julie Lenkei, Zsolt Zala, Diana
author_facet	Tasso, Mariana Pons, Thomas Lequeux, Nicolas Nguyen, Julie Lenkei, Zsolt Zala, Diana
author_sort	Tasso, Mariana
title	NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
title_short	NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
title_full	NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
title_fullStr	NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
title_full_unstemmed	NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
title_sort	nanopaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/127302
work_keys_str_mv	AT tassomariana nanopaintatoolforrapidanddynamicimagingofmembranestructuralplasticityatthenanoscale AT ponsthomas nanopaintatoolforrapidanddynamicimagingofmembranestructuralplasticityatthenanoscale AT lequeuxnicolas nanopaintatoolforrapidanddynamicimagingofmembranestructuralplasticityatthenanoscale AT nguyenjulie nanopaintatoolforrapidanddynamicimagingofmembranestructuralplasticityatthenanoscale AT lenkeizsolt nanopaintatoolforrapidanddynamicimagingofmembranestructuralplasticityatthenanoscale AT zaladiana nanopaintatoolforrapidanddynamicimagingofmembranestructuralplasticityatthenanoscale
bdutipo_str	Repositorios
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NanoPaint: a tool for rapid and dynamic imaging of membrane structural plasticity at the nanoscale

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