Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers

Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect...

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Autores principales:	Henning, María Florencia, Sánchez, Susana A., Bakás, Laura Susana
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2009
Materias:	Bioquímica Membrane raft Giant unilamellar vesicles Two-photon microscopy LAURDANFITC-LPS Detergent solubilization Triton X-100 LAURDAN generalized polarization Cholesterol Phase segregation
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/128273
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-128273
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Bioquímica Membrane raft Giant unilamellar vesicles Two-photon microscopy LAURDANFITC-LPS Detergent solubilization Triton X-100 LAURDAN generalized polarization Cholesterol Phase segregation
spellingShingle	Bioquímica Membrane raft Giant unilamellar vesicles Two-photon microscopy LAURDANFITC-LPS Detergent solubilization Triton X-100 LAURDAN generalized polarization Cholesterol Phase segregation Henning, María Florencia Sánchez, Susana A. Bakás, Laura Susana Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
topic_facet	Bioquímica Membrane raft Giant unilamellar vesicles Two-photon microscopy LAURDANFITC-LPS Detergent solubilization Triton X-100 LAURDAN generalized polarization Cholesterol Phase segregation
description	Lipopolysaccharide (LPS) is an endotoxin released from the outer membrane of Gram-negative bacteria during infections. It have been reported that LPS may play a role in the outer membrane of bacteria similar to that of cholesterol in eukaryotic plasma membranes. In this article we compare the effect of introducing LPS or cholesterol in liposomes made of dipalmitoylphosphatidylcholine/dioleoylphosphatidylcholine on the solubilization process by Triton X-100. The results show that liposomes containing LPS or cholesterol are more resistant to solubilization by Triton X-100 than the binary phospholipid mixtures at 4 °C. The LPS distribution was analyzed on GUVs of DPPC:DOPC using FITC-LPS. Solid and liquid-crystalline domains were visualized labeling the GUVs with LAURDAN and GP images were acquired using a two-photon microscope. The images show a selective distribution of LPS in gel domains. Our results support the hypothesis that LPS could aggregate and concentrate selectively in biological membranes providing a mechanism to bring together several components of the LPS-sensing machinery.
format	Articulo Articulo
author	Henning, María Florencia Sánchez, Susana A. Bakás, Laura Susana
author_facet	Henning, María Florencia Sánchez, Susana A. Bakás, Laura Susana
author_sort	Henning, María Florencia
title	Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_short	Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_full	Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_fullStr	Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_full_unstemmed	Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
title_sort	visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers
publishDate	2009
url	http://sedici.unlp.edu.ar/handle/10915/128273
work_keys_str_mv	AT henningmariaflorencia visualizationandanalysisoflipopolysaccharidedistributioninbinaryphospholipidbilayers AT sanchezsusanaa visualizationandanalysisoflipopolysaccharidedistributioninbinaryphospholipidbilayers AT bakaslaurasusana visualizationandanalysisoflipopolysaccharidedistributioninbinaryphospholipidbilayers
bdutipo_str	Repositorios
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Visualization and analysis of lipopolysaccharide distribution in binary phospholipid bilayers

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