Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina

The genetic structure and diversity of <i>Mycosphaerella graminicola</i> population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Provi...

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Detalles Bibliográficos
Autores principales: Castillo, Nadia, Cordo, Cristina Alicia, Simón, María Rosa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131456
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131456
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
spellingShingle Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
Castillo, Nadia
Cordo, Cristina Alicia
Simón, María Rosa
Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
topic_facet Ciencias Agrarias
Dendrogram
Genetic diversity
Haplotypes
ISSR
Population
description The genetic structure and diversity of <i>Mycosphaerella graminicola</i> population were studied with ISSR molecular markers, using isolates from several locations of the Argentinean wheat region: subregion IV (SE of Buenos Aires Province) and II South (central part of Buenos Aires Province). Samples were taken from different bread wheat (<i>Triticum aestivum</i>) cultivars. A total of 126 isolates were subjected to molecular analysis to compare the genetic structure of the isolates from both wheat subregions. Ten ISSR primers were used: (GACA)₄; (AAC)₇; (ATC)₇; (AC)₉; (AAG)₇; (AG)₉; (AGC)₅; (CAG)₅, (GTG)₅ and (GACAC)₃. Eighty-four bands ranging from 200 bp to 8.000 were amplified. Eighty-one distinct haplotypes were identified and 43 isolates did not generate any amplification products. The highest number of polymorphic DNA fragments were produced using ISSR primers (ATC)₇ and (GTG)₅, which detected bands in 38 isolates. The molecular analysis revealed the existence of 81 different haplotypes among the 126 isolates studied. These results revealed a high degree of genetic diversity in the <i>M. graminicola</i> population in Argentina.
format Articulo
Articulo
author Castillo, Nadia
Cordo, Cristina Alicia
Simón, María Rosa
author_facet Castillo, Nadia
Cordo, Cristina Alicia
Simón, María Rosa
author_sort Castillo, Nadia
title Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_short Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_full Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_fullStr Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_full_unstemmed Molecular variability among isolates of <i>Mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in Argentina
title_sort molecular variability among isolates of <i>mycosphaerella graminicola</i>, the causal agent of septoria tritici blotch, in argentina
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/131456
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