Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)

Leptolegnia chapmanii is an aquatic fungus that has demonstrated marked pathogenicity towards the larvae of a number of mosquito species with little or no effect on non-target insects. The aim of this work was to determine the best culture medium for colony growth, and production and infectivity of...

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Detalles Bibliográficos
Autores principales:	Pelizza, Sebastián Alberto, Cabello, Marta Noemí, Tranchida, María Cecilia, Scorsetti, Ana Clara, Bisaro, Vilma
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Ciencias Naturales Ciencias Agrarias Leptolegnia chapmanii Isolate Culture medium Aedes aegypti
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/131877
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-131877
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Ciencias Agrarias Leptolegnia chapmanii Isolate Culture medium Aedes aegypti
spellingShingle	Ciencias Naturales Ciencias Agrarias Leptolegnia chapmanii Isolate Culture medium Aedes aegypti Pelizza, Sebastián Alberto Cabello, Marta Noemí Tranchida, María Cecilia Scorsetti, Ana Clara Bisaro, Vilma Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
topic_facet	Ciencias Naturales Ciencias Agrarias Leptolegnia chapmanii Isolate Culture medium Aedes aegypti
description	Leptolegnia chapmanii is an aquatic fungus that has demonstrated marked pathogenicity towards the larvae of a number of mosquito species with little or no effect on non-target insects. The aim of this work was to determine the best culture medium for colony growth, and production and infectivity of zoospores among different isolates of L. chapmanii. No significant differences (P >0.01) were observed for the media FORT(agar), PYG, YPSS, and PYGSF in terms of colony diameter of the different isolates evaluated. In these culture media, all L. chapmanii isolates had the highest growth (45 mm2) after 7 days. The nine isolates tested produced the greatest number of zoospores in FORT(agar) medium 3 (1 × 106 zoospores/ml). Medium YPSS exhibited significant differences (P <0.0001) compared to other culture media with respect to maintenance of virulence and infectivity of the isolates, producing 100% larval mortality after 48 h of inoculation. Thus, for the cultivation and maintenance of L. chapmanii isolates, the medium of choice among those tested is FORT(agar), as it is both inexpensive to prepare and ensures optimal colony development, extremely high zoospore production, and excellent maintenance of fungal infectivity.
format	Articulo Articulo
author	Pelizza, Sebastián Alberto Cabello, Marta Noemí Tranchida, María Cecilia Scorsetti, Ana Clara Bisaro, Vilma
author_facet	Pelizza, Sebastián Alberto Cabello, Marta Noemí Tranchida, María Cecilia Scorsetti, Ana Clara Bisaro, Vilma
author_sort	Pelizza, Sebastián Alberto
title	Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_short	Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_full	Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_fullStr	Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_full_unstemmed	Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
title_sort	screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of leptolegnia chapmanii (straminipila: peronosporomycetes)
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/131877
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Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)

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