RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer

In previous studies, we identified rhomboid domain containing 2 (RHBDD2) gene to be markedly overexpressed in breast cancer patients that developed recurrence of the disease. In this study, we evaluated for the first time RHBDD2 gene expression in colorectal cancer (CRC). Five public available DNA m...

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Autores principales: Lacunza, Ezequiel, Canzoneri, Romina, Rabassa, Martín Enrique, Zwenger, Ariel Osvaldo, Segal-Eiras, Amada, Croce, María Virginia, Abba, Martín Carlos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/133492
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-133492
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
spellingShingle Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
Lacunza, Ezequiel
Canzoneri, Romina
Rabassa, Martín Enrique
Zwenger, Ariel Osvaldo
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
topic_facet Medicina
Colorectal cancer
RHBDD2 gene expression
5FU treatment
description In previous studies, we identified rhomboid domain containing 2 (RHBDD2) gene to be markedly overexpressed in breast cancer patients that developed recurrence of the disease. In this study, we evaluated for the first time RHBDD2 gene expression in colorectal cancer (CRC). Five public available DNA microarray studies were compiled in a homogeneous dataset of 906 colorectal samples. The statistical analysis of these data showed a significant increase of RHBDD2 expression in the advanced stages of CRC (p < 0.01). We validated these findings by immunohistochemistry on 130 colorectal tissue samples; RHBDD2 protein overexpression was also observed in the advanced stages of the disease (p < 0.001). In addition, we investigated RHBDD2 expression in response to the chemotherapy agent 5-fluorouracile (5FU). We detected a significant increase of RHBDD2 mRNA and protein after 5FU treatment (20–40 μM; p < 0.001). Overall, these results showed that RHBDD2 overexpression might play a role in colorectal cancer progression.
format Articulo
Articulo
author Lacunza, Ezequiel
Canzoneri, Romina
Rabassa, Martín Enrique
Zwenger, Ariel Osvaldo
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
author_facet Lacunza, Ezequiel
Canzoneri, Romina
Rabassa, Martín Enrique
Zwenger, Ariel Osvaldo
Segal-Eiras, Amada
Croce, María Virginia
Abba, Martín Carlos
author_sort Lacunza, Ezequiel
title RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_short RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_full RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_fullStr RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_full_unstemmed RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
title_sort rhbdd2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/133492
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bdutipo_str Repositorios
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