Desaturase Activities in Rat Model of Insulin Resistance Induced by a Sucrose-Rich Diet

A sucrose-rich diet, as compared with a similar starch diet, induces a time-dependent typical noninsulin-dependent diabetes syndrome characterized by insulin resistance in rats. Within the first 3 wk, there was glucose intolerance associated with hyperinsulinemia, hypertriglyceridemia, and high plas...

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Autores principales: Brenner, Rodolfo Roberto, Rimoldi, Omar Jorge, Lombardo, Yolanda B., González, María Susana, Bernasconi, Ana María, Chicco, Adriana, Basabe, Juan C.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Medicina
insulin-dependent diabetes mellitus
insulin resistance
sucrose-rich diet
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/133878
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-133878
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
insulin-dependent diabetes mellitus
insulin resistance
sucrose-rich diet
spellingShingle Medicina
insulin-dependent diabetes mellitus
insulin resistance
sucrose-rich diet
Brenner, Rodolfo Roberto
Rimoldi, Omar Jorge
Lombardo, Yolanda B.
González, María Susana
Bernasconi, Ana María
Chicco, Adriana
Basabe, Juan C.
Desaturase Activities in Rat Model of Insulin Resistance Induced by a Sucrose-Rich Diet
topic_facet Medicina
insulin-dependent diabetes mellitus
insulin resistance
sucrose-rich diet
description A sucrose-rich diet, as compared with a similar starch diet, induces a time-dependent typical noninsulin-dependent diabetes syndrome characterized by insulin resistance in rats. Within the first 3 wk, there was glucose intolerance associated with hyperinsulinemia, hypertriglyceridemia, and high plasma FFA. In this study, we examined the effect of the sucrose-rich diet vs. the starch diet during short-(3 wk) and long-term treatment (6 mon) on hepatic Δ9, Δ6, and Δ5 desaturases. These enzymes modulate monounsaturated FA and PUFA biosynthesis, respectively. Sucrose feeding (3 wk) caused an initial hyperinsulinemia that was normalized within 6 mon. In the early period (3 wk), stearoyl-CoA desaturase-1 (SCD-1) mRNA and activity were decreased, whereas Δ6 desaturase mRNA abundance and Δ6 and Δ5 desaturase activities remained unchanged. After 6 mon of sucrose feeding, activities of the Δ9, Δ6, and Δ5 desaturases were each increased. The SCD-1 and Δ6 desaturase mRNA were also correspondingly higher. These increases were consistent with an increase in oleic acid, the 20∶4/18∶2 ratio, and 22∶4n−6 and 22∶5n−6 acids in liver and muscle lipids. On the other hand, the percentage of 22∶6n−3 acid was decreased. In conclusion, a sucrose-rich diet after 6 mon induces an increase in rat liver SCD-1 and Δ6 desaturase mRNA and enzymatic activities that are opposite to the changes reported in insulin-dependent diabetes mellitus. It appears that neither blood insulin levels nor insulin resistance is a factor affecting the Δ9, Δ6, and Δ5 desaturase changes in mRNA and activity found with the sucrose-rich diet.
format Articulo
Articulo
author Brenner, Rodolfo Roberto
Rimoldi, Omar Jorge
Lombardo, Yolanda B.
González, María Susana
Bernasconi, Ana María
Chicco, Adriana
Basabe, Juan C.
author_facet Brenner, Rodolfo Roberto
Rimoldi, Omar Jorge
Lombardo, Yolanda B.
González, María Susana
Bernasconi, Ana María
Chicco, Adriana
Basabe, Juan C.
author_sort Brenner, Rodolfo Roberto
title Desaturase Activities in Rat Model of Insulin Resistance Induced by a Sucrose-Rich Diet
title_short Desaturase Activities in Rat Model of Insulin Resistance Induced by a Sucrose-Rich Diet
title_full Desaturase Activities in Rat Model of Insulin Resistance Induced by a Sucrose-Rich Diet
title_fullStr Desaturase Activities in Rat Model of Insulin Resistance Induced by a Sucrose-Rich Diet
title_full_unstemmed Desaturase Activities in Rat Model of Insulin Resistance Induced by a Sucrose-Rich Diet
title_sort desaturase activities in rat model of insulin resistance induced by a sucrose-rich diet
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/133878
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