Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets
Fatty acid binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids and cholesterol de novo, FABPs, together with other lipid binding proteins, have been proposed as essenti...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2020
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/136766 |
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I19-R120-10915-1367662023-11-08T14:15:35Z http://sedici.unlp.edu.ar/handle/10915/136766 Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets Bélgamo, Julián Alberto Alberca, Lucas Nicolás Pórfido, Jorge Luis Caram Romero, Franco Nahuel Rodríguez, Santiago Talevi, Alan Córsico, Betina Franchini, Gisela Raquel 2020 2022-05-23T19:05:59Z en Bioquímica Drug repurposing Target repurposing FABP Echinococcus spp Virtual screening Neglected tropical diseases Fatty acid binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids and cholesterol de novo, FABPs, together with other lipid binding proteins, have been proposed as essential, involved in the trafficking and delivery of such lipophilic metabolites. Pharmacological agents that modify specific parasite FABP function may provide control of lipid signalling pathways, inflammatory responses and metabolic regulation that could be of crucial importance for the parasite development and survival. Echinococcus multilocularis and Echinococcus granulosus are, respectively, the causative agents of alveolar and cystic echinococcosis (or hydatidosis). These diseases are included in the World Health Organization's list of priority neglected tropical diseases. Here, we explore the potential of FABPs from cestodes as drug targets. To this end, we have applied a target repurposing approach to identify novel inhibitors of Echinococcus spp. FABPs. An ensemble of computational models was developed and applied in a virtual screening campaign of DrugBank library. 21 hits belonging to the applicability domain of the ensemble models were identified, and 3 of the hits were assayed against purified E. multilocularis FABP, experimentally confirming the model's predictions. Noteworthy, this is to our best knowledge the first report on isolation and purification of such four FABP, for which initial structural and functional characterization is reported here. Instituto de Investigaciones Bioquímicas de La Plata Laboratorio de Investigación y Desarrollo de Bioactivos Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 1275-1288 |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Drug repurposing Target repurposing FABP Echinococcus spp Virtual screening Neglected tropical diseases |
| spellingShingle |
Bioquímica Drug repurposing Target repurposing FABP Echinococcus spp Virtual screening Neglected tropical diseases Bélgamo, Julián Alberto Alberca, Lucas Nicolás Pórfido, Jorge Luis Caram Romero, Franco Nahuel Rodríguez, Santiago Talevi, Alan Córsico, Betina Franchini, Gisela Raquel Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets |
| topic_facet |
Bioquímica Drug repurposing Target repurposing FABP Echinococcus spp Virtual screening Neglected tropical diseases |
| description |
Fatty acid binding proteins (FABPs) are small intracellular proteins that reversibly bind fatty acids and other hydrophobic ligands. In cestodes, due to their inability to synthesise fatty acids and cholesterol de novo, FABPs, together with other lipid binding proteins, have been proposed as essential, involved in the trafficking and delivery of such lipophilic metabolites. Pharmacological agents that modify specific parasite FABP function may provide control of lipid signalling pathways, inflammatory responses and metabolic regulation that could be of crucial importance for the parasite development and survival. Echinococcus multilocularis and Echinococcus granulosus are, respectively, the causative agents of alveolar and cystic echinococcosis (or hydatidosis). These diseases are included in the World Health Organization's list of priority neglected tropical diseases. Here, we explore the potential of FABPs from cestodes as drug targets. To this end, we have applied a target repurposing approach to identify novel inhibitors of Echinococcus spp. FABPs. An ensemble of computational models was developed and applied in a virtual screening campaign of DrugBank library. 21 hits belonging to the applicability domain of the ensemble models were identified, and 3 of the hits were assayed against purified E. multilocularis FABP, experimentally confirming the model's predictions. Noteworthy, this is to our best knowledge the first report on isolation and purification of such four FABP, for which initial structural and functional characterization is reported here. |
| format |
Articulo Articulo |
| author |
Bélgamo, Julián Alberto Alberca, Lucas Nicolás Pórfido, Jorge Luis Caram Romero, Franco Nahuel Rodríguez, Santiago Talevi, Alan Córsico, Betina Franchini, Gisela Raquel |
| author_facet |
Bélgamo, Julián Alberto Alberca, Lucas Nicolás Pórfido, Jorge Luis Caram Romero, Franco Nahuel Rodríguez, Santiago Talevi, Alan Córsico, Betina Franchini, Gisela Raquel |
| author_sort |
Bélgamo, Julián Alberto |
| title |
Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets |
| title_short |
Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets |
| title_full |
Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets |
| title_fullStr |
Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets |
| title_full_unstemmed |
Application of target repositioning and in silico screening to exploit fatty acid binding proteins (FABPs) from Echinococcus multilocularis as possible drug targets |
| title_sort |
application of target repositioning and in silico screening to exploit fatty acid binding proteins (fabps) from echinococcus multilocularis as possible drug targets |
| publishDate |
2020 |
| url |
http://sedici.unlp.edu.ar/handle/10915/136766 |
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