Yeasts from kefir grains: isolation, identification, and probiotic characterization

Kefir—a traditional beverage whose consumption has been associated with health benefits—is a logical natural product to investigate for new probiotic strains. The aim of the present work was to isolate and identify kefir yeasts and select those with acid and bile tolerance to study their adhesion to...

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Detalles Bibliográficos
Autores principales: Diosma, Gabriela, Romanin, David Emmanuel, Rey Burusco, María Florencia, Londero, Alejandra, Garrote, Graciela Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Biología
Ciencias Agrarias
Probiotics
Yeasts
Saccharomyces
Kluyveromyces
Kefir
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139392
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139392
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Agrarias
Probiotics
Yeasts
Saccharomyces
Kluyveromyces
Kefir
spellingShingle Biología
Ciencias Agrarias
Probiotics
Yeasts
Saccharomyces
Kluyveromyces
Kefir
Diosma, Gabriela
Romanin, David Emmanuel
Rey Burusco, María Florencia
Londero, Alejandra
Garrote, Graciela Liliana
Yeasts from kefir grains: isolation, identification, and probiotic characterization
topic_facet Biología
Ciencias Agrarias
Probiotics
Yeasts
Saccharomyces
Kluyveromyces
Kefir
description Kefir—a traditional beverage whose consumption has been associated with health benefits—is a logical natural product to investigate for new probiotic strains. The aim of the present work was to isolate and identify kefir yeasts and select those with acid and bile tolerance to study their adhesion to epithelial cells and their transit through mouse gut. From 4 milky and 3 sugary kefir grains, 34 yeast strains were isolated and identified by means of classical microbiological and molecular-genetic methods (whole-cell protein pattern, internal-transcribed-spacer amplification, and analysis of restriction-fragment–length polymorphisms). We identified 4 species belonging to 3 genera—<i>Saccharomyces cerevisiae</i> (15 strains), <i>Saccharomyces unisporus</i> (6 strains), <i>Issatchenkia occidentalis</i> (4 strains), and <i>Kluyveromyces marxianus</i> (9 strains)—and selected 13 strains on the basis of resistance to low pH and bile salts. Among the strains selected, <i>Kluyveromyces marxianus</i> CIDCA 8154 and <i>Saccharomyces cerevisiae</i> CIDCA 8112 were further studied. Both strains evidenced the capacity to adhere to epithelial intestine-derived cells in vitro and to survive passage through the gastrointestinal tract of BALB/c mice. The investigation of the potential probiotic features of these kefir-yeast strains should be useful for the development of novel functional foods.
format Articulo
Articulo
author Diosma, Gabriela
Romanin, David Emmanuel
Rey Burusco, María Florencia
Londero, Alejandra
Garrote, Graciela Liliana
author_facet Diosma, Gabriela
Romanin, David Emmanuel
Rey Burusco, María Florencia
Londero, Alejandra
Garrote, Graciela Liliana
author_sort Diosma, Gabriela
title Yeasts from kefir grains: isolation, identification, and probiotic characterization
title_short Yeasts from kefir grains: isolation, identification, and probiotic characterization
title_full Yeasts from kefir grains: isolation, identification, and probiotic characterization
title_fullStr Yeasts from kefir grains: isolation, identification, and probiotic characterization
title_full_unstemmed Yeasts from kefir grains: isolation, identification, and probiotic characterization
title_sort yeasts from kefir grains: isolation, identification, and probiotic characterization
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/139392
work_keys_str_mv AT diosmagabriela yeastsfromkefirgrainsisolationidentificationandprobioticcharacterization
AT romanindavidemmanuel yeastsfromkefirgrainsisolationidentificationandprobioticcharacterization
AT reyburuscomariaflorencia yeastsfromkefirgrainsisolationidentificationandprobioticcharacterization
AT londeroalejandra yeastsfromkefirgrainsisolationidentificationandprobioticcharacterization
AT garrotegracielaliliana yeastsfromkefirgrainsisolationidentificationandprobioticcharacterization
bdutipo_str Repositorios
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