Effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (PHB) content and molecular weight (Mw) from Azotobacter chroococcum 6B

The molecular weight (Mw) of poly-3-hydroxybutyrate (PHB), produced by shake-flask culture of Azotobacter chroococcum showed little variation with increasing glucose concentration as carbon source (being in the range of 400–500 kDa), while M; w increased from 300–400 to 640 kDa when grown with incre...

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Detalles Bibliográficos
Autores principales: Quagliano, J. C., Amarilla. F., Fernandes, E. G., Mata, D., Miyazaki, S. S.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Química
Ciencias Exactas
Azotobacter chroococcum 6B
culture temperature
molasses
molecular weight
poly-3-hydroxybutyrate
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/142162
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-142162
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Ciencias Exactas
Azotobacter chroococcum 6B
culture temperature
molasses
molecular weight
poly-3-hydroxybutyrate
spellingShingle Química
Ciencias Exactas
Azotobacter chroococcum 6B
culture temperature
molasses
molecular weight
poly-3-hydroxybutyrate
Quagliano, J. C.
Amarilla. F.
Fernandes, E. G.
Mata, D.
Miyazaki, S. S.
Effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (PHB) content and molecular weight (Mw) from Azotobacter chroococcum 6B
topic_facet Química
Ciencias Exactas
Azotobacter chroococcum 6B
culture temperature
molasses
molecular weight
poly-3-hydroxybutyrate
description The molecular weight (Mw) of poly-3-hydroxybutyrate (PHB), produced by shake-flask culture of Azotobacter chroococcum showed little variation with increasing glucose concentration as carbon source (being in the range of 400–500 kDa), while M; w increased from 300–400 to 640 kDa when grown with increasing concentration of sugar cane molasses. Molecular weight increased nearly 30% from 48 to 72 h culture time when 5% molasses as carbon source was used, while with glucose the highest M; w was reached at 48 h. Under fermentor cultivation A. chroococcum produced PHB with a relatively high M; w of 1590 kDa at 53 h culture time when grown in modified Burk's medium with glucose as carbon source at an initial C/N ratio (molar basis) of 69 under fermentor cultivation. A batch glucose-grown ammonium-limited fermentor culture was repeatedly fed with sugar cane molasses (initial C/N ratio 69) and it was observed that PHB content curve decreased at a slower rate than in the fed-batch culture in which glucose and sucrose were not consumed in the culture medium after the feed.
format Articulo
Articulo
author Quagliano, J. C.
Amarilla. F.
Fernandes, E. G.
Mata, D.
Miyazaki, S. S.
author_facet Quagliano, J. C.
Amarilla. F.
Fernandes, E. G.
Mata, D.
Miyazaki, S. S.
author_sort Quagliano, J. C.
title Effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (PHB) content and molecular weight (Mw) from Azotobacter chroococcum 6B
title_short Effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (PHB) content and molecular weight (Mw) from Azotobacter chroococcum 6B
title_full Effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (PHB) content and molecular weight (Mw) from Azotobacter chroococcum 6B
title_fullStr Effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (PHB) content and molecular weight (Mw) from Azotobacter chroococcum 6B
title_full_unstemmed Effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (PHB) content and molecular weight (Mw) from Azotobacter chroococcum 6B
title_sort effect of simple and complex carbon sources, low temperature culture and complex carbon feeding policies on poly-3-hydroxybutyric acid (phb) content and molecular weight (mw) from azotobacter chroococcum 6b
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/142162
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