HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization

Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacryla...

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Autores principales: Torres, María Luz, Oberti, Tamara Gisela, Fernández, Juan Manuel
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2021
Materias:
Química
Semi-interpenetrating polymer network
Chondrocyte
Chondrocompatibility
Swelling
Contact angle
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/143199
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-143199
record_format dspace
spelling I19-R120-10915-1431992023-12-04T14:58:23Z http://sedici.unlp.edu.ar/handle/10915/143199 HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization Torres, María Luz Oberti, Tamara Gisela Fernández, Juan Manuel 2021-01-12 2022-10-03T16:14:22Z en Química Semi-interpenetrating polymer network Chondrocyte Chondrocompatibility Swelling Contact angle Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE. Laboratorio de Investigación en Osteopatías y Metabolismo Mineral Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Preprint http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 504-523
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Semi-interpenetrating polymer network
Chondrocyte
Chondrocompatibility
Swelling
Contact angle
spellingShingle Química
Semi-interpenetrating polymer network
Chondrocyte
Chondrocompatibility
Swelling
Contact angle
Torres, María Luz
Oberti, Tamara Gisela
Fernández, Juan Manuel
HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization
topic_facet Química
Semi-interpenetrating polymer network
Chondrocyte
Chondrocompatibility
Swelling
Contact angle
description Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE.
format Articulo
Preprint
author Torres, María Luz
Oberti, Tamara Gisela
Fernández, Juan Manuel
author_facet Torres, María Luz
Oberti, Tamara Gisela
Fernández, Juan Manuel
author_sort Torres, María Luz
title HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization
title_short HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization
title_full HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization
title_fullStr HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization
title_full_unstemmed HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization
title_sort hema and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/143199
work_keys_str_mv AT torresmarialuz hemaandalginatebasedchondrogenicsemiinterpenetratedhydrogelssynthesisandbiologicalcharacterization
AT obertitamaragisela hemaandalginatebasedchondrogenicsemiinterpenetratedhydrogelssynthesisandbiologicalcharacterization
AT fernandezjuanmanuel hemaandalginatebasedchondrogenicsemiinterpenetratedhydrogelssynthesisandbiologicalcharacterization
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