New Media for Increasing Sporulation and Germination of <i>Phaeoisariopsis griseola</i> Conidia

The slow growth rate of the fungus Phaeoisariopsis griseola and the availability of a homogeneous highly concentrated inoculum is an important constraint for pathogenicity or virulence studies, where plant inoculations are needed. Therefore, the purpose of this paper was to evaluate the effect of su...

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Autores principales: Stenglein, Sebastián Alberto, Aulicino, Mónica Beatriz, Arambarri, Ana María, Balatti, Pedro Alberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Agrarias
Amaranthus cruentus
Angular leaf spot
Supplemented media
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145118
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145118
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Amaranthus cruentus
Angular leaf spot
Supplemented media
spellingShingle Ciencias Agrarias
Amaranthus cruentus
Angular leaf spot
Supplemented media
Stenglein, Sebastián Alberto
Aulicino, Mónica Beatriz
Arambarri, Ana María
Balatti, Pedro Alberto
New Media for Increasing Sporulation and Germination of <i>Phaeoisariopsis griseola</i> Conidia
topic_facet Ciencias Agrarias
Amaranthus cruentus
Angular leaf spot
Supplemented media
description The slow growth rate of the fungus Phaeoisariopsis griseola and the availability of a homogeneous highly concentrated inoculum is an important constraint for pathogenicity or virulence studies, where plant inoculations are needed. Therefore, the purpose of this paper was to evaluate the effect of supplementing culture media with Amaranthus cruentus seed meal on fungal growth and sporulation of isolates of P. griseola belonging to the Mesoamerican and Andean groups. The amendment of PDA or V8 media with A. cruentus seed meal resulted in a considerable increase in the number of conidia and also in their capacity to germinate; this depended mostly on the stage of maturity of conidia. Mesoamerican and Andean isolates produced a different number of conidia when cultured in vitro. Furthermore, while in Mesoamerican isolates a second degree polynomial represented the relationship between number of conidia and amount of A. cruentus supplementation, in Andean isolates the relationship was linear. It seems that either one or several of the nutritional factors provided by A. cruentus contributed to the increased production of conidia and their development, resulting in faster development of the disease and an earlier appearance of symptoms. Therefore, for cultural studies, especially for inoculum production and for pathogenicity evaluations, supplementation of the media with A. cruentus seed meal proved to be a good alternative.
format Articulo
Articulo
author Stenglein, Sebastián Alberto
Aulicino, Mónica Beatriz
Arambarri, Ana María
Balatti, Pedro Alberto
author_facet Stenglein, Sebastián Alberto
Aulicino, Mónica Beatriz
Arambarri, Ana María
Balatti, Pedro Alberto
author_sort Stenglein, Sebastián Alberto
title New Media for Increasing Sporulation and Germination of <i>Phaeoisariopsis griseola</i> Conidia
title_short New Media for Increasing Sporulation and Germination of <i>Phaeoisariopsis griseola</i> Conidia
title_full New Media for Increasing Sporulation and Germination of <i>Phaeoisariopsis griseola</i> Conidia
title_fullStr New Media for Increasing Sporulation and Germination of <i>Phaeoisariopsis griseola</i> Conidia
title_full_unstemmed New Media for Increasing Sporulation and Germination of <i>Phaeoisariopsis griseola</i> Conidia
title_sort new media for increasing sporulation and germination of <i>phaeoisariopsis griseola</i> conidia
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/145118
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