Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain

The stomach-derived hormone ghrelin mainly acts in the brain. Studies in mice have shown that the accessibility of ghrelin into the brain is limited and that it mainly takes place in some circumventricular organs, such as the median eminence. Notably, some known brain targets of ghrelin are distantl...

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Detalles Bibliográficos
Autores principales: Uriarte Donati, Maia, De Francesco, Pablo Nicolás, Fernández, Gimena, Cabral, Agustina Soledad, Castrogiovanni, Daniel Cayetano, Lalonde, Tyler, Luyt, Leonard G., Trejo, Sebastián Alejandro, Perelló, Mario Carlos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Biología
Ependymal cells
Choroid plexus
Tanycytes
Hypothalamus
Ghrelin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/145491
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-145491
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ependymal cells
Choroid plexus
Tanycytes
Hypothalamus
Ghrelin
spellingShingle Biología
Ependymal cells
Choroid plexus
Tanycytes
Hypothalamus
Ghrelin
Uriarte Donati, Maia
De Francesco, Pablo Nicolás
Fernández, Gimena
Cabral, Agustina Soledad
Castrogiovanni, Daniel Cayetano
Lalonde, Tyler
Luyt, Leonard G.
Trejo, Sebastián Alejandro
Perelló, Mario Carlos
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
topic_facet Biología
Ependymal cells
Choroid plexus
Tanycytes
Hypothalamus
Ghrelin
description The stomach-derived hormone ghrelin mainly acts in the brain. Studies in mice have shown that the accessibility of ghrelin into the brain is limited and that it mainly takes place in some circumventricular organs, such as the median eminence. Notably, some known brain targets of ghrelin are distantly located from the circumventricular organs. Thus, we hypothesized that ghrelin could also access the brain via the blood-cerebrospinal fluid (CSF) barrier, which consists of the choroid plexus and the hypothalamic tanycytes. Using systemic injection of ghrelin or fluorescent-ghrelin in mice, we found that cells of the blood-CSF barrier internalize these molecules. In time-response studies, we found that peripherally injected fluorescent-ghrelin quickly reaches hypothalamic regions located in apposition to the median eminence and more slowly reaches the periventricular hypothalamic parenchyma, adjacent to the dorsal part of the third ventricle. Additionally, we found that CSF ghrelin levels increase after the systemic administration of ghrelin, and that central infusions of either an anti-ghrelin antibody, which immuno-neutralizes CSF ghrelin, or a scrambled version of ghrelin, which is also internalized by cells of the blood-CSF barrier, partially impair the orexigenic effect of peripherally injected ghrelin. Thus, current evidence suggests that the blood-CSF barrier can transport circulating ghrelin into the brain, and that the access of ghrelin into the CSF is required for its full orexigenic effect.
format Articulo
Articulo
author Uriarte Donati, Maia
De Francesco, Pablo Nicolás
Fernández, Gimena
Cabral, Agustina Soledad
Castrogiovanni, Daniel Cayetano
Lalonde, Tyler
Luyt, Leonard G.
Trejo, Sebastián Alejandro
Perelló, Mario Carlos
author_facet Uriarte Donati, Maia
De Francesco, Pablo Nicolás
Fernández, Gimena
Cabral, Agustina Soledad
Castrogiovanni, Daniel Cayetano
Lalonde, Tyler
Luyt, Leonard G.
Trejo, Sebastián Alejandro
Perelló, Mario Carlos
author_sort Uriarte Donati, Maia
title Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
title_short Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
title_full Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
title_fullStr Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
title_full_unstemmed Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
title_sort evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/145491
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