Triggered release of proteins from emulsan–alginate beads
Emulsan/alginate beads were studied for protein adsorption and stability in the context of controlled release. The beads, 400F80 Am diameter with approximately 10% emulsan content, offer unusual opportunities for delivery of proteins due to the natural ability of emulsan to bind proteins, coupled wi...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2005
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/153292 |
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I19-R120-10915-1532922023-05-19T20:08:22Z http://sedici.unlp.edu.ar/handle/10915/153292 issn:0168-3659 issn:1873-4995 Triggered release of proteins from emulsan–alginate beads Castro, Guillermo Raúl Kamdar, Romit R. Panilaitis, Bruce Kaplan, David L. 2005 2023-05-19T16:45:05Z en Bioquímica Emulsan/alginate beads controlled release Emulsan/alginate beads were studied for protein adsorption and stability in the context of controlled release. The beads, 400F80 Am diameter with approximately 10% emulsan content, offer unusual opportunities for delivery of proteins due to the natural ability of emulsan to bind proteins, coupled with the selective biological activation features of this complex lipoheteropolysaccharide. The binding capacity of azo-bovine serum albumin by the emulsan/alginate beads was 0.637 ± 0.004 vs. 0.170 ± 0.007 μg/mg for beads formed from alginate alone. In additional protein adsorption experiments, the lipase and subtilisin maintained activity when adsorbed to the emulsan/alginate beads albeit with lower specific activity when compared to the enzyme free in solution. However, the half life of the adsorbed enzyme was significantly higher than the free forms. To explore functional utility of this system, two types of triggered release were studied in the context of these bead systems. First, azo-BSA as a model protein was physically bound to emulsan/alginate beads and then selectively released by triggering with subtilisin, a serine protease, which cleaves the azo dye, sulfanilic acid, from the bound protein. In absence of subtilisin no triggered release was observed. Second, azo-BSA as a prodrug model, was adsorbed to the emulsan/alginate beads and then release of the dye was demonstrated by lipase treatment which cleaves the fatty acid esters from the emulsan structure to release the bound protein. The results establish the versatility and utility of emulsan-based beads for protein binding and triggered release. Centro de Investigación y Desarrollo en Fermentaciones Industriales Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 149-157 |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Emulsan/alginate beads controlled release |
| spellingShingle |
Bioquímica Emulsan/alginate beads controlled release Castro, Guillermo Raúl Kamdar, Romit R. Panilaitis, Bruce Kaplan, David L. Triggered release of proteins from emulsan–alginate beads |
| topic_facet |
Bioquímica Emulsan/alginate beads controlled release |
| description |
Emulsan/alginate beads were studied for protein adsorption and stability in the context of controlled release. The beads, 400F80 Am diameter with approximately 10% emulsan content, offer unusual opportunities for delivery of proteins due to the natural ability of emulsan to bind proteins, coupled with the selective biological activation features of this complex lipoheteropolysaccharide. The binding capacity of azo-bovine serum albumin by the emulsan/alginate beads was 0.637 ± 0.004 vs. 0.170 ± 0.007 μg/mg for beads formed from alginate alone. In additional protein adsorption experiments, the lipase and subtilisin maintained activity when adsorbed to the emulsan/alginate beads albeit with lower specific activity when compared to the enzyme free in solution. However, the half life of the adsorbed enzyme was significantly higher than the free forms. To explore functional utility of this system, two types of triggered release were studied in the context of these bead systems. First, azo-BSA as a model protein was physically bound to emulsan/alginate beads and then selectively released by triggering with subtilisin, a serine protease, which cleaves the azo dye, sulfanilic acid, from the bound protein. In absence of subtilisin no triggered release was observed. Second, azo-BSA as a prodrug model, was adsorbed to the emulsan/alginate beads and then release of the dye was demonstrated by lipase treatment which cleaves the fatty acid esters from the emulsan structure to release the bound protein. The results establish the versatility and utility of emulsan-based beads for protein binding and triggered release. |
| format |
Articulo Articulo |
| author |
Castro, Guillermo Raúl Kamdar, Romit R. Panilaitis, Bruce Kaplan, David L. |
| author_facet |
Castro, Guillermo Raúl Kamdar, Romit R. Panilaitis, Bruce Kaplan, David L. |
| author_sort |
Castro, Guillermo Raúl |
| title |
Triggered release of proteins from emulsan–alginate beads |
| title_short |
Triggered release of proteins from emulsan–alginate beads |
| title_full |
Triggered release of proteins from emulsan–alginate beads |
| title_fullStr |
Triggered release of proteins from emulsan–alginate beads |
| title_full_unstemmed |
Triggered release of proteins from emulsan–alginate beads |
| title_sort |
triggered release of proteins from emulsan–alginate beads |
| publishDate |
2005 |
| url |
http://sedici.unlp.edu.ar/handle/10915/153292 |
| work_keys_str_mv |
AT castroguillermoraul triggeredreleaseofproteinsfromemulsanalginatebeads AT kamdarromitr triggeredreleaseofproteinsfromemulsanalginatebeads AT panilaitisbruce triggeredreleaseofproteinsfromemulsanalginatebeads AT kaplandavidl triggeredreleaseofproteinsfromemulsanalginatebeads |
| _version_ |
1766999717977260032 |