In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows

Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO)...

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Autores principales: Buldain, Daniel Cornelio, Gortari Castillo, Lihuel, Buchamer, Andrea Verónica, Bandoni, Arnaldo Luis, Marchetti, María Laura, Mestorino, Olga Nora
Formato: Articulo
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/153603
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-153603
record_format dspace
spelling I19-R120-10915-1536032023-05-29T20:09:17Z http://sedici.unlp.edu.ar/handle/10915/153603 issn:2297-1769 In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows Buldain, Daniel Cornelio Gortari Castillo, Lihuel Buchamer, Andrea Verónica Bandoni, Arnaldo Luis Marchetti, María Laura Mestorino, Olga Nora 2022-11-15 2023-05-29T18:49:58Z en Ciencias Veterinarias Melaleuca armillaris essential oil erythromycin synergism Staphylococcus aureus mastitis Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus. Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
spellingShingle Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo Luis
Marchetti, María Laura
Mestorino, Olga Nora
In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
topic_facet Ciencias Veterinarias
Melaleuca armillaris
essential oil
erythromycin
synergism
Staphylococcus aureus
mastitis
description Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.
format Articulo
Articulo
author Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo Luis
Marchetti, María Laura
Mestorino, Olga Nora
author_facet Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Bandoni, Arnaldo Luis
Marchetti, María Laura
Mestorino, Olga Nora
author_sort Buldain, Daniel Cornelio
title In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_short In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_full In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_fullStr In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_full_unstemmed In vitro synergistic interaction between <i>Melaleuca armillaris</i> essential oil and erythromycin against <i>Staphylococcus aureus</i> isolated from dairy cows
title_sort in vitro synergistic interaction between <i>melaleuca armillaris</i> essential oil and erythromycin against <i>staphylococcus aureus</i> isolated from dairy cows
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/153603
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