The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha

Traditional methods of clearing and mounting geophilomorph centipedes have proved to be unsatisfactory. Both 60 per cent lactic acid and lactophenol, which are often used for temporary mounts, do not clear specimens adequately and cause slight swelling of the appendages, altering their shape. In add...

Descripción completa

Detalles Bibliográficos
Autor principal: Pereira, Luis Alberto
Formato: Articulo
Lenguaje:Español
Publicado: 2000
Materias:
Ciencias Naturales
Geofilomorfos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/156522
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-156522
record_format dspace
spelling I19-R120-10915-1565222023-08-18T04:07:27Z http://sedici.unlp.edu.ar/handle/10915/156522 The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha Pereira, Luis Alberto 2000 2023-08-17T16:23:50Z es Ciencias Naturales Geofilomorfos Traditional methods of clearing and mounting geophilomorph centipedes have proved to be unsatisfactory. Both 60 per cent lactic acid and lactophenol, which are often used for temporary mounts, do not clear specimens adequately and cause slight swelling of the appendages, altering their shape. In addition, lactophenol contains glycerol which is hygroscopic and therefore takes up some water from the air, which is an additional disadvantage. Many workers, for example Attems and Verhoeff, made permanent mounts, macerating specimens by heating them in 10 per cent potassium hydroxide, washing, then dehydrating in a series of alcohols. They were cleared in xylene and mounted in Canada balsam. Potassium hydroxide, however, damages poorly sclerotized structures as well as the internal organs which are important for classification (for example the structure of the coxal organs) and also for assessing the maturity of the specimen (presence of spermatozoa in the seminiferous tubules of males and spermathecae of females). In addition specimens treated with potassium hydroxide become deformed under the pressure of the cover glass when mounted. Facultad de Ciencias Naturales y Museo Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 22-25
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Naturales
Geofilomorfos
spellingShingle Ciencias Naturales
Geofilomorfos
Pereira, Luis Alberto
The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha
topic_facet Ciencias Naturales
Geofilomorfos
description Traditional methods of clearing and mounting geophilomorph centipedes have proved to be unsatisfactory. Both 60 per cent lactic acid and lactophenol, which are often used for temporary mounts, do not clear specimens adequately and cause slight swelling of the appendages, altering their shape. In addition, lactophenol contains glycerol which is hygroscopic and therefore takes up some water from the air, which is an additional disadvantage. Many workers, for example Attems and Verhoeff, made permanent mounts, macerating specimens by heating them in 10 per cent potassium hydroxide, washing, then dehydrating in a series of alcohols. They were cleared in xylene and mounted in Canada balsam. Potassium hydroxide, however, damages poorly sclerotized structures as well as the internal organs which are important for classification (for example the structure of the coxal organs) and also for assessing the maturity of the specimen (presence of spermatozoa in the seminiferous tubules of males and spermathecae of females). In addition specimens treated with potassium hydroxide become deformed under the pressure of the cover glass when mounted.
format Articulo
Articulo
author Pereira, Luis Alberto
author_facet Pereira, Luis Alberto
author_sort Pereira, Luis Alberto
title The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha
title_short The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha
title_full The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha
title_fullStr The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha
title_full_unstemmed The preparation of centipedes for microscopical examination with particular reference to the Geophilomorpha
title_sort preparation of centipedes for microscopical examination with particular reference to the geophilomorpha
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/156522
work_keys_str_mv AT pereiraluisalberto thepreparationofcentipedesformicroscopicalexaminationwithparticularreferencetothegeophilomorpha
AT pereiraluisalberto preparationofcentipedesformicroscopicalexaminationwithparticularreferencetothegeophilomorpha
_version_ 1807221025019002880

Ejemplares similares