Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data

When subjected to molecular study, species of digeneans believed to be cosmopolitan are usually found to consist of complexes of species with narrower distributions. We present molecular and morphological evidence of transcontinental distributions in two species of Apharyngostrigea Ciurea, 1924, bas...

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Autores principales: Locke, Sean A., Drago, Fabiana Beatriz, López Hernández, Danimar, Chibwana, Fred D., Núñez, María Verónica, Van Dam, Alex, Achinelly, María Fernanda, Johnson, Pieter T. J., Costa Alves de Assis, Jordana, Lane de Melo, Alan, Alves Pinto, Hudson
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160226
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id I19-R120-10915-160226
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spelling I19-R120-10915-1602262023-11-15T20:07:51Z http://sedici.unlp.edu.ar/handle/10915/160226 Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data Locke, Sean A. Drago, Fabiana Beatriz López Hernández, Danimar Chibwana, Fred D. Núñez, María Verónica Van Dam, Alex Achinelly, María Fernanda Johnson, Pieter T. J. Costa Alves de Assis, Jordana Lane de Melo, Alan Alves Pinto, Hudson 2021 2023-11-15T18:40:44Z en Ciencias Naturales Helminth Trematoda Biogeography Heron Macroparasite Northern leopard frog Diplostomoidea When subjected to molecular study, species of digeneans believed to be cosmopolitan are usually found to consist of complexes of species with narrower distributions. We present molecular and morphological evidence of transcontinental distributions in two species of Apharyngostrigea Ciurea, 1924, based on samples from Africa and the Americas. Sequences of cytochrome c oxidase I and, in some samples, internal transcribed spacer, revealed Apharyngostrigea pipientis (Faust, 1918) in Tanzania (first known African record), Argentina, Brazil, USA and Canada. Sequences from A. pipientis also match previously published sequences identified as Apharyngostrigea cornu (Zeder, 1800) originating in Mexico. Hosts of A. pipientis surveyed include definitive hosts from the Afrotropic, Neotropic and Nearctic, as well as first and second intermediate hosts from the Americas, including the type host and type region. In addition, metacercariae of A. pipientis were obtained from experimentally infected Poecilia reticulata, the first known record of this parasite in a non-amphibian second intermediate host. Variation in cytochrome c oxidase I haplotypes in A. pipientis is consistent with a long established, wide-ranging species with moderate genetic structure among Nearctic, Neotropic and Afrotropic regions. We attribute this to natural dispersal by birds and find no evidence of anthropogenic introductions of exotic host species. Sequences of CO1 and ITS from adult Apharyngostrigea simplex (Johnston, 1904) from Egretta thula in Argentina matched published data from cercariae from Biomphalaria straminea from Brazil and metacercariae from Cnesterodon decemmaculatus in Argentina, consistent with previous morphological and life-cycle studies reporting this parasite—originally described in Australia—in South America. Analyses of the mitochondrial genome and rDNA operon from A. pipientis support prior phylogenies based on shorter markers showing the Strigeidae Railliet, 1919 to be polyphyletic. Facultad de Ciencias Naturales y Museo Centro de Estudios Parasitológicos y de Vectores Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 667-683
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Helminth
Trematoda
Biogeography
Heron
Macroparasite
Northern leopard frog
Diplostomoidea
spellingShingle Ciencias Naturales
Helminth
Trematoda
Biogeography
Heron
Macroparasite
Northern leopard frog
Diplostomoidea
Locke, Sean A.
Drago, Fabiana Beatriz
López Hernández, Danimar
Chibwana, Fred D.
Núñez, María Verónica
Van Dam, Alex
Achinelly, María Fernanda
Johnson, Pieter T. J.
Costa Alves de Assis, Jordana
Lane de Melo, Alan
Alves Pinto, Hudson
Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data
topic_facet Ciencias Naturales
Helminth
Trematoda
Biogeography
Heron
Macroparasite
Northern leopard frog
Diplostomoidea
description When subjected to molecular study, species of digeneans believed to be cosmopolitan are usually found to consist of complexes of species with narrower distributions. We present molecular and morphological evidence of transcontinental distributions in two species of Apharyngostrigea Ciurea, 1924, based on samples from Africa and the Americas. Sequences of cytochrome c oxidase I and, in some samples, internal transcribed spacer, revealed Apharyngostrigea pipientis (Faust, 1918) in Tanzania (first known African record), Argentina, Brazil, USA and Canada. Sequences from A. pipientis also match previously published sequences identified as Apharyngostrigea cornu (Zeder, 1800) originating in Mexico. Hosts of A. pipientis surveyed include definitive hosts from the Afrotropic, Neotropic and Nearctic, as well as first and second intermediate hosts from the Americas, including the type host and type region. In addition, metacercariae of A. pipientis were obtained from experimentally infected Poecilia reticulata, the first known record of this parasite in a non-amphibian second intermediate host. Variation in cytochrome c oxidase I haplotypes in A. pipientis is consistent with a long established, wide-ranging species with moderate genetic structure among Nearctic, Neotropic and Afrotropic regions. We attribute this to natural dispersal by birds and find no evidence of anthropogenic introductions of exotic host species. Sequences of CO1 and ITS from adult Apharyngostrigea simplex (Johnston, 1904) from Egretta thula in Argentina matched published data from cercariae from Biomphalaria straminea from Brazil and metacercariae from Cnesterodon decemmaculatus in Argentina, consistent with previous morphological and life-cycle studies reporting this parasite—originally described in Australia—in South America. Analyses of the mitochondrial genome and rDNA operon from A. pipientis support prior phylogenies based on shorter markers showing the Strigeidae Railliet, 1919 to be polyphyletic.
format Articulo
Articulo
author Locke, Sean A.
Drago, Fabiana Beatriz
López Hernández, Danimar
Chibwana, Fred D.
Núñez, María Verónica
Van Dam, Alex
Achinelly, María Fernanda
Johnson, Pieter T. J.
Costa Alves de Assis, Jordana
Lane de Melo, Alan
Alves Pinto, Hudson
author_facet Locke, Sean A.
Drago, Fabiana Beatriz
López Hernández, Danimar
Chibwana, Fred D.
Núñez, María Verónica
Van Dam, Alex
Achinelly, María Fernanda
Johnson, Pieter T. J.
Costa Alves de Assis, Jordana
Lane de Melo, Alan
Alves Pinto, Hudson
author_sort Locke, Sean A.
title Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data
title_short Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data
title_full Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data
title_fullStr Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data
title_full_unstemmed Intercontinental distributions, phylogenetic position and life cycles of species of Apharyngostrigea (Digenea, Diplostomoidea) illuminated with morphological, experimental, molecular and genomic data
title_sort intercontinental distributions, phylogenetic position and life cycles of species of apharyngostrigea (digenea, diplostomoidea) illuminated with morphological, experimental, molecular and genomic data
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/160226
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