Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action

The aim of this study is to show that introducing a small fraction of hydrophilic groups into a hydrophobic polyester favor the macrophage activity by accelerating the degradation action in aqueous media. It is also seen that differentiation of MSCs cultured in monolayer towards bone in specific dif...

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Autores principales: Fernández, Juan Manuel, Oberti, Tamara Gisela, Vikingsson, Line, Gómez Ribelles, José Luis, Cortizo, Ana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Química
Biología
polycaprolactone
poly(L-lactide)
poly(hydroxyethyl acrylate)
macrophage erosion
enzymatic degradation
mesenchymal stem cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/163980
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-163980
record_format dspace
spelling I19-R120-10915-1639802024-03-19T04:07:19Z http://sedici.unlp.edu.ar/handle/10915/163980 Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action Fernández, Juan Manuel Oberti, Tamara Gisela Vikingsson, Line Gómez Ribelles, José Luis Cortizo, Ana María 2016-08 2024-03-18T18:08:01Z en Química Biología polycaprolactone poly(L-lactide) poly(hydroxyethyl acrylate) macrophage erosion enzymatic degradation mesenchymal stem cells The aim of this study is to show that introducing a small fraction of hydrophilic groups into a hydrophobic polyester favor the macrophage activity by accelerating the degradation action in aqueous media. It is also seen that differentiation of MSCs cultured in monolayer towards bone in specific differentiation media is favored in these materials with respect to the corresponding pristine polyesters. Polymer networks based in polycarpolactone or poly(L-lactide) and containing a small fraction of poly(-hydroxyethyl acrylate) have been synthesized. Degradation kinetics "in vitro" was monitored by mass loss and swelling capacity of the polymer network in good solvents, the later as representative of chain cleavage. Hydrolytic and enzymatic degradation is accelerated by the inclusion of poly(hydroxyethyl acrylate) blocks in the network. Macrophages were cultured on the surface of the network films, showing its capacity to erode the material surface but also to accelerate bulk degradation. Bone marrow mesenchymal stem cells were cultured in monolayer on the membranes in osteogenic media, showing an increase of specific markers expression in comparison to pristine polyesters. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Laboratorio de Investigación en Osteopatías y Metabolismo Mineral Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 38-46
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biología
polycaprolactone
poly(L-lactide)
poly(hydroxyethyl acrylate)
macrophage erosion
enzymatic degradation
mesenchymal stem cells
spellingShingle Química
Biología
polycaprolactone
poly(L-lactide)
poly(hydroxyethyl acrylate)
macrophage erosion
enzymatic degradation
mesenchymal stem cells
Fernández, Juan Manuel
Oberti, Tamara Gisela
Vikingsson, Line
Gómez Ribelles, José Luis
Cortizo, Ana María
Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action
topic_facet Química
Biología
polycaprolactone
poly(L-lactide)
poly(hydroxyethyl acrylate)
macrophage erosion
enzymatic degradation
mesenchymal stem cells
description The aim of this study is to show that introducing a small fraction of hydrophilic groups into a hydrophobic polyester favor the macrophage activity by accelerating the degradation action in aqueous media. It is also seen that differentiation of MSCs cultured in monolayer towards bone in specific differentiation media is favored in these materials with respect to the corresponding pristine polyesters. Polymer networks based in polycarpolactone or poly(L-lactide) and containing a small fraction of poly(-hydroxyethyl acrylate) have been synthesized. Degradation kinetics "in vitro" was monitored by mass loss and swelling capacity of the polymer network in good solvents, the later as representative of chain cleavage. Hydrolytic and enzymatic degradation is accelerated by the inclusion of poly(hydroxyethyl acrylate) blocks in the network. Macrophages were cultured on the surface of the network films, showing its capacity to erode the material surface but also to accelerate bulk degradation. Bone marrow mesenchymal stem cells were cultured in monolayer on the membranes in osteogenic media, showing an increase of specific markers expression in comparison to pristine polyesters.
format Articulo
Articulo
author Fernández, Juan Manuel
Oberti, Tamara Gisela
Vikingsson, Line
Gómez Ribelles, José Luis
Cortizo, Ana María
author_facet Fernández, Juan Manuel
Oberti, Tamara Gisela
Vikingsson, Line
Gómez Ribelles, José Luis
Cortizo, Ana María
author_sort Fernández, Juan Manuel
title Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action
title_short Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action
title_full Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action
title_fullStr Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action
title_full_unstemmed Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action
title_sort biodegradable polyester networks including hydrophilic groups favor bmscs differentiation and can be eroded by macrophage action
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/163980
work_keys_str_mv AT fernandezjuanmanuel biodegradablepolyesternetworksincludinghydrophilicgroupsfavorbmscsdifferentiationandcanbeerodedbymacrophageaction
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AT gomezribellesjoseluis biodegradablepolyesternetworksincludinghydrophilicgroupsfavorbmscsdifferentiationandcanbeerodedbymacrophageaction
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