Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy

Photothermal therapy (PTT) is a noninvasive treatment for cancer relying on the incorporation of NIRlight absorbing nanomaterials into cells, which upon illumination release heat causing thermally induced cell death. We prove that irradiation of aqueous suspensions of poly(vinylpyrrolidone)-coated s...

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Autores principales:	Rivas Aiello, María Belén, Azcárate, Julio César, Zelaya Soulé, María Emilia, David Gara, Pedro Maximiliano, Bosio, Gabriela Natalia, Gensch, Thomas, Mártire, Daniel Osvaldo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2021
Materias:	Física Química photothermal therapy HeLa cells
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/178524
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-178524
record_format	dspace
spelling	I19-R120-10915-1785242025-04-28T20:11:47Z http://sedici.unlp.edu.ar/handle/10915/178524 Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy Rivas Aiello, María Belén Azcárate, Julio César Zelaya Soulé, María Emilia David Gara, Pedro Maximiliano Bosio, Gabriela Natalia Gensch, Thomas Mártire, Daniel Osvaldo 2021-01-01 2025-04-28T12:24:35Z en Física Química photothermal therapy HeLa cells Photothermal therapy (PTT) is a noninvasive treatment for cancer relying on the incorporation of NIRlight absorbing nanomaterials into cells, which upon illumination release heat causing thermally induced cell death. We prove that irradiation of aqueous suspensions of poly(vinylpyrrolidone)-coated silver nanoplates (PVPAgNP) or PVPAgNP in HeLa cells with red or NIR lasers causes a sizeable photothermal effect, which in cells can be visualized with the temperature sensing fluorophore Rhodamine B (RhB) using spinning disk confocal fluorescence microscopy or fluorescence lifetime imaging. Upon red-light irradiation of cells that were incubated with both, RhB and PVPAgNP at concentrations with no adverse effects on cell viability, a substantial heat release is detected. Initiation of cell death by photothermal effect is observed by positive signals of fluorescent markers for early and late apoptosis. Surprisingly, a new nanomaterial- assisted cell killing mode is operating when PVPAgNP-loaded HeLa cells are excited with moderate powers of fs-pulsed NIR light. Small roundish areas are generated with bright and fast (<1 ns) decaying emission, which expand fast and destroy the whole cell in seconds. This characteristic emission is assigned to efficient optical breakdown initiation around the strongly absorbing PVPAgNP leading to plasma formation that spreads fast through the cell. Laboratorio de Ablación Láser, Fotónica e Imágenes 3D Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Centro de Investigaciones Ópticas Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf 2608-2619
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Física Química photothermal therapy HeLa cells
spellingShingle	Física Química photothermal therapy HeLa cells Rivas Aiello, María Belén Azcárate, Julio César Zelaya Soulé, María Emilia David Gara, Pedro Maximiliano Bosio, Gabriela Natalia Gensch, Thomas Mártire, Daniel Osvaldo Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy
topic_facet	Física Química photothermal therapy HeLa cells
description	Photothermal therapy (PTT) is a noninvasive treatment for cancer relying on the incorporation of NIRlight absorbing nanomaterials into cells, which upon illumination release heat causing thermally induced cell death. We prove that irradiation of aqueous suspensions of poly(vinylpyrrolidone)-coated silver nanoplates (PVPAgNP) or PVPAgNP in HeLa cells with red or NIR lasers causes a sizeable photothermal effect, which in cells can be visualized with the temperature sensing fluorophore Rhodamine B (RhB) using spinning disk confocal fluorescence microscopy or fluorescence lifetime imaging. Upon red-light irradiation of cells that were incubated with both, RhB and PVPAgNP at concentrations with no adverse effects on cell viability, a substantial heat release is detected. Initiation of cell death by photothermal effect is observed by positive signals of fluorescent markers for early and late apoptosis. Surprisingly, a new nanomaterial- assisted cell killing mode is operating when PVPAgNP-loaded HeLa cells are excited with moderate powers of fs-pulsed NIR light. Small roundish areas are generated with bright and fast (<1 ns) decaying emission, which expand fast and destroy the whole cell in seconds. This characteristic emission is assigned to efficient optical breakdown initiation around the strongly absorbing PVPAgNP leading to plasma formation that spreads fast through the cell.
format	Articulo Articulo
author	Rivas Aiello, María Belén Azcárate, Julio César Zelaya Soulé, María Emilia David Gara, Pedro Maximiliano Bosio, Gabriela Natalia Gensch, Thomas Mártire, Daniel Osvaldo
author_facet	Rivas Aiello, María Belén Azcárate, Julio César Zelaya Soulé, María Emilia David Gara, Pedro Maximiliano Bosio, Gabriela Natalia Gensch, Thomas Mártire, Daniel Osvaldo
author_sort	Rivas Aiello, María Belén
title	Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy
title_short	Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy
title_full	Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy
title_fullStr	Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy
title_full_unstemmed	Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy
title_sort	photothermal therapy with silver nanoplates in hela cells studied by in situ fluorescence microscopy
publishDate	2021
url	http://sedici.unlp.edu.ar/handle/10915/178524
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Photothermal therapy with silver nanoplates in HeLa cells studied by in situ fluorescence microscopy

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