An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study

A simple and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for the determination of auraptene (AUR) in dog plasma. The chromatographic separation of AUR was achieved on a C18 column using isocratic elution with acetonitrile-0.1 % formic acid (volume rat...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Li, Ming, Yuan, Jinbin, Chen, Heifang, Yang, Wuliang, Luo, Xiaoquan, Liu, Yingin, Zhang, Yu
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
auraptene; pharmacokinetics; HPLC; dog plasma
Cromatografía Liquida
Cromatografía
Técnicas de Química Analítica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/18099
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_12.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-18099
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
auraptene; pharmacokinetics; HPLC; dog plasma
Cromatografía Liquida
Cromatografía
Técnicas de Química Analítica
spellingShingle Farmacia
auraptene; pharmacokinetics; HPLC; dog plasma
Cromatografía Liquida
Cromatografía
Técnicas de Química Analítica
Li, Ming
Yuan, Jinbin
Chen, Heifang
Yang, Wuliang
Luo, Xiaoquan
Liu, Yingin
Zhang, Yu
An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study
topic_facet Farmacia
auraptene; pharmacokinetics; HPLC; dog plasma
Cromatografía Liquida
Cromatografía
Técnicas de Química Analítica
description A simple and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for the determination of auraptene (AUR) in dog plasma. The chromatographic separation of AUR was achieved on a C18 column using isocratic elution with acetonitrile-0.1 % formic acid (volume ratio 84.5:15.5). AUR was detected at 322 nm. Sample extraction with ethyl acetate resulted in high recoveries of AUR. A linear curve over the concentration range 10.2-408.8 ng/mL (r2 = 0.9995) was obtained. Satisfactory intra-day and inter-day precisions were achieved with RSDs less than 9.5 % and the average recovery factors were in the range of 99.1-106.2 %. The method was used to determine the plasma concentration–time profiles for AUR after oral doses of 50, 100 and 200 mg/kg in dogs. A nonlinear pharmacokinetics was found in dogs at doses from 50 to 200 mg/kg. No significant accumulation of AUR in dogs following multiple doses was observed.
format Articulo
Articulo
author Li, Ming
Yuan, Jinbin
Chen, Heifang
Yang, Wuliang
Luo, Xiaoquan
Liu, Yingin
Zhang, Yu
author_facet Li, Ming
Yuan, Jinbin
Chen, Heifang
Yang, Wuliang
Luo, Xiaoquan
Liu, Yingin
Zhang, Yu
author_sort Li, Ming
title An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study
title_short An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study
title_full An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study
title_fullStr An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study
title_full_unstemmed An HPLC Method for the Determination of Auraptene in Dog Plasma: Application to Pharmacokinetic Study
title_sort hplc method for the determination of auraptene in dog plasma: application to pharmacokinetic study
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/18099
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_12.pdf
work_keys_str_mv AT liming anhplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT yuanjinbin anhplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT chenheifang anhplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT yangwuliang anhplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT luoxiaoquan anhplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT liuyingin anhplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT zhangyu anhplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT liming hplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT yuanjinbin hplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT chenheifang hplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT yangwuliang hplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT luoxiaoquan hplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT liuyingin hplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
AT zhangyu hplcmethodforthedeterminationofaurapteneindogplasmaapplicationtopharmacokineticstudy
bdutipo_str Repositorios
_version_ 1764820463802908672

Ejemplares similares