HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP

In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gen...

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Autores principales: Abba, Martín Carlos, Gómez, María Atilia, Golijow, Carlos Daniel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Veterinarias
agar gel electrophoresis
HLA-DQA1
LIS-SSCP
genetics
PCR
osmolarity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/37574
http://www.scielo.br/pdf/bjmbr/v34n7/4121.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-37574
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
agar gel electrophoresis
HLA-DQA1
LIS-SSCP
genetics
PCR
osmolarity
spellingShingle Ciencias Veterinarias
agar gel electrophoresis
HLA-DQA1
LIS-SSCP
genetics
PCR
osmolarity
Abba, Martín Carlos
Gómez, María Atilia
Golijow, Carlos Daniel
HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
topic_facet Ciencias Veterinarias
agar gel electrophoresis
HLA-DQA1
LIS-SSCP
genetics
PCR
osmolarity
description In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a small native polyacrylamide gel, followed by a rapid silver staining procedure. In order to validate the technique and to obtain the allele patterns for the DQA1 gene, 50 cervical samples were typed using this methodology and the commercial Amplitype® HLA DQA1 Amplification and Typing kit. All the alleles detected with the kit were characterized by the LIS-SSCP approach. This procedure proved to be useful for population screening and typing of the DQA1 gene as well as for detecting new alleles or mutations in the donor-recipient molecular matching of HLA class II genes.
format Articulo
Articulo
author Abba, Martín Carlos
Gómez, María Atilia
Golijow, Carlos Daniel
author_facet Abba, Martín Carlos
Gómez, María Atilia
Golijow, Carlos Daniel
author_sort Abba, Martín Carlos
title HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
title_short HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
title_full HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
title_fullStr HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
title_full_unstemmed HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP
title_sort hla-dqa1 allele typing by nonisotopic pcr-lis-sscp
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/37574
http://www.scielo.br/pdf/bjmbr/v34n7/4121.pdf
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