Structure and interactions of fish type III antifreeze protein in solution

It has been suggested that above a critical protein concentration, fish Type III antifreeze protein (AFP III) selfassembles to form micelle-like structures that may play a key role In antifreeze activity. To understand the complex activity of AFP III, a comprehensive description of its association s...

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Detalles Bibliográficos
Autores principales:	Salvay, Andrés Gerardo, Gabel, Frank, Pucci, Bernard, Santos, Javier, Howards, Eduardo, Ebel, Christine
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Física Air Animals Antifreeze Proteins, Type III Chromatography, Gel Light Models, Molecular Molecular Weight Neutron Diffraction Scattering, Radiation Scattering, Small Angle Solutions Surface Tension Ultracentrifugation Water
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82477
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82477
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Física Air Animals Antifreeze Proteins, Type III Chromatography, Gel Light Models, Molecular Molecular Weight Neutron Diffraction Scattering, Radiation Scattering, Small Angle Solutions Surface Tension Ultracentrifugation Water
spellingShingle	Física Air Animals Antifreeze Proteins, Type III Chromatography, Gel Light Models, Molecular Molecular Weight Neutron Diffraction Scattering, Radiation Scattering, Small Angle Solutions Surface Tension Ultracentrifugation Water Salvay, Andrés Gerardo Gabel, Frank Pucci, Bernard Santos, Javier Howards, Eduardo Ebel, Christine Structure and interactions of fish type III antifreeze protein in solution
topic_facet	Física Air Animals Antifreeze Proteins, Type III Chromatography, Gel Light Models, Molecular Molecular Weight Neutron Diffraction Scattering, Radiation Scattering, Small Angle Solutions Surface Tension Ultracentrifugation Water
description	It has been suggested that above a critical protein concentration, fish Type III antifreeze protein (AFP III) selfassembles to form micelle-like structures that may play a key role In antifreeze activity. To understand the complex activity of AFP III, a comprehensive description of its association state and structural organization in solution is necessary. We used analytical ultracentrifugation, analytical size-exclusion chromatography, and dynamic light scattering to characterize the interactions and homogeneity of AFP III in solution. Small-angle neutron scattering was used to determine the low-resolution structure in solution. Our results clearly show that at concentrations up to 20 mg mL-1 and at temperatures of 20°C, 6°C, and 4°C, AFP III is monomeric in solution and adopts a structure compatible with that determined by crystallography. Surface tension measurements show a propensity of AFP III to localize at the air/water interface, but this surface activity is not correlated with any aggregation in the bulk. These results support the hypothesis that each AFP III molecule acts independently of the others, and that specific intermolecular interactions between monomers are not required for binding to ice. The lack of attractive interactions between monomers may be functionally important, allowing for more efficient binding and covering of the ice surface.
format	Articulo Articulo
author	Salvay, Andrés Gerardo Gabel, Frank Pucci, Bernard Santos, Javier Howards, Eduardo Ebel, Christine
author_facet	Salvay, Andrés Gerardo Gabel, Frank Pucci, Bernard Santos, Javier Howards, Eduardo Ebel, Christine
author_sort	Salvay, Andrés Gerardo
title	Structure and interactions of fish type III antifreeze protein in solution
title_short	Structure and interactions of fish type III antifreeze protein in solution
title_full	Structure and interactions of fish type III antifreeze protein in solution
title_fullStr	Structure and interactions of fish type III antifreeze protein in solution
title_full_unstemmed	Structure and interactions of fish type III antifreeze protein in solution
title_sort	structure and interactions of fish type iii antifreeze protein in solution
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/82477
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bdutipo_str	Repositorios
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Structure and interactions of fish type III antifreeze protein in solution

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