Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum

<b>Context:</b> Hedeoma multiflorum Benth. (Lamiaceae) is widely used in Argentinean popular medicine for digestive and anti-spasmodic purposes. However, knowledge about its pharmacological properties has been poorly investigated. <b>Objective:</b> The antioxidant and cytot...

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Autores principales: Dadé, Martín Miguel, Schinella, Guillermo Raúl, Fioravanti, Daniel Emilio, Tournier, Horacio Alfio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Biología
Farmacia
Apoptosis
Hedeoma multiflorum
Lipid peroxidation
polymorphonuclear neutrophils
scavenger
Peroxidación de Lípido
Carroñero
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84290
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84290
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Farmacia
Apoptosis
Hedeoma multiflorum
Lipid peroxidation
polymorphonuclear neutrophils
scavenger
Peroxidación de Lípido
Carroñero
spellingShingle Biología
Farmacia
Apoptosis
Hedeoma multiflorum
Lipid peroxidation
polymorphonuclear neutrophils
scavenger
Peroxidación de Lípido
Carroñero
Dadé, Martín Miguel
Schinella, Guillermo Raúl
Fioravanti, Daniel Emilio
Tournier, Horacio Alfio
Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum
topic_facet Biología
Farmacia
Apoptosis
Hedeoma multiflorum
Lipid peroxidation
polymorphonuclear neutrophils
scavenger
Peroxidación de Lípido
Carroñero
description <b>Context:</b> Hedeoma multiflorum Benth. (Lamiaceae) is widely used in Argentinean popular medicine for digestive and anti-spasmodic purposes. However, knowledge about its pharmacological properties has been poorly investigated. <b>Objective:</b> The antioxidant and cytotoxic properties of an aqueous extract from the plant were investigated for the first time. <b>Material and methods:</b> Scavenging of stable free radicals of 2,2-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS+) and 2,2-diphenyl-1-picryl hydrazyl (DPPH), reducing of ferric (III) iron of ferric reducing ability of plasma (FRAP) reagent, and inhibition of lipid peroxidation (LP) of human plasma and rat brain homogenates were assessed. Cytotoxicity was tested on human polymorphonuclear (PMN) cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. Cytotoxicity was assessed by flow cytometric techniques. <b>Results:</b> Extract scavenged ABTS+ and DPPH (1.78 and 0.78 Âμ mol Trolox equivalent/mg dry extract, respectively) and reduced FRAP reagent (0.66 μmol ascorbic acid equivalent/mg dry extract). LP of human plasma and rat brain was also inhibited in a dose-dependent way (inhibitory concentration 50%=27.0 and 86.0 μg/mL, respectively). Extract is rich in polyphenol compounds (0.96±0.08 μmol equivalent caffeic acid/mg dry matter). Treatment of PMN decreased significantly the cell ability to reduce the MTT salt and increased the hypodiploid nuclei from 4 to 18% quantified using propidium iodide (PI). In the annexin V-Fluorescein isothiocyanate (annexin V-FITC) assay, 26% of treated cells were annexin V-FITC positive and PI negative. Using the 3,3′-dihexyloxacarbocyanine iodide uptake method, the negative fraction of cells was calculated as 29%. <b>Discussion and conclusion:</b> H. multiflorum extract was found to have a significant antioxidant and pro-apoptotic activities, and a great potential as a source of healthy products.
format Articulo
Articulo
author Dadé, Martín Miguel
Schinella, Guillermo Raúl
Fioravanti, Daniel Emilio
Tournier, Horacio Alfio
author_facet Dadé, Martín Miguel
Schinella, Guillermo Raúl
Fioravanti, Daniel Emilio
Tournier, Horacio Alfio
author_sort Dadé, Martín Miguel
title Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum
title_short Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum
title_full Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum
title_fullStr Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum
title_full_unstemmed Antioxidant and cytotoxic properties of an aqueous extract from the Argentinean plant Hedeoma multiflorum
title_sort antioxidant and cytotoxic properties of an aqueous extract from the argentinean plant hedeoma multiflorum
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/84290
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