Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line

Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to opti...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Crispo, Martina, Schlapp, Geraldine, Cárdenas Rodríguez, Magdalena, González Maciel, María Dolores, Rumbo, Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Exactas
DNA concentration
Microinjection
Reporter systems
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85303
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85303
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
DNA concentration
Microinjection
Reporter systems
spellingShingle Ciencias Exactas
DNA concentration
Microinjection
Reporter systems
Crispo, Martina
Schlapp, Geraldine
Cárdenas Rodríguez, Magdalena
González Maciel, María Dolores
Rumbo, Martín
Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
topic_facet Ciencias Exactas
DNA concentration
Microinjection
Reporter systems
description Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to optimize aspects of the procedure. Three different concentrations (0.5, 1.0 and 4.0 ng/μl) of the DNA construction to microinject a total of 1981 zygotes has been tested. Intact/injected embryos, pregnancy and birth rate, survival of pups 7 days after birth, number of transgenic pups and overall transgenic efficiency was registered and analyzed by Z test of proportions for each group. Results: A total of seven transgenic founders were detected for the three DNA concentrations used, 1 in 46 alive pups in the 0.5 ng/μl group, 5 in 38 alive pups in the 1 ng/μl group and 1 in 21 alive pups in the 4 ng/μl group (p < 0.1). The overall transgenic efficiency was higher for the 1 ng/μl concentration, with a transgenic rate of 13.2%. Conclusions: In conclusion, we have selected the best operative conditions to maximize the transgenesis efficiency. Furthermore, the transgenic lines developed could be used as a reporter model of innate immunity activation with many different applications in the fields of immunology, cancer and neurodegenerative diseases.
format Articulo
Articulo
author Crispo, Martina
Schlapp, Geraldine
Cárdenas Rodríguez, Magdalena
González Maciel, María Dolores
Rumbo, Martín
author_facet Crispo, Martina
Schlapp, Geraldine
Cárdenas Rodríguez, Magdalena
González Maciel, María Dolores
Rumbo, Martín
author_sort Crispo, Martina
title Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
title_short Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
title_full Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
title_fullStr Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
title_full_unstemmed Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
title_sort optimization of transgenesis conditions for the generation of cxcl2-luciferase reporter mice line
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85303
work_keys_str_mv AT crispomartina optimizationoftransgenesisconditionsforthegenerationofcxcl2luciferasereportermiceline
AT schlappgeraldine optimizationoftransgenesisconditionsforthegenerationofcxcl2luciferasereportermiceline
AT cardenasrodriguezmagdalena optimizationoftransgenesisconditionsforthegenerationofcxcl2luciferasereportermiceline
AT gonzalezmacielmariadolores optimizationoftransgenesisconditionsforthegenerationofcxcl2luciferasereportermiceline
AT rumbomartin optimizationoftransgenesisconditionsforthegenerationofcxcl2luciferasereportermiceline
bdutipo_str Repositorios
_version_ 1764820489230876673

Ejemplares similares