Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes

In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe2+ (2.15 μM) system...

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Autores principales: Palacios, Alejandro, Barberón, Javier, Leaden, Patricio, Zeinsteger, Pedro Adolfo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85835
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85835
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
spellingShingle Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
Palacios, Alejandro
Barberón, Javier
Leaden, Patricio
Zeinsteger, Pedro Adolfo
Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
topic_facet Ciencias Veterinarias
Chemiluminescence
Lipoperoxidation
Marigold
description In this study the effects of Calendula officinalis L. (Asteraceae) extract (CO) on the polyunsaturated fatty acid composition, chemiluminescence and unsaturation index of microsomes isolated from brain rat, are presented. After incubation of microsomes in an ascorbate (0.4 mM)-Fe2+ (2.15 μM) system (180 min at 37 °C) it was observed that the total cpm/mg protein originated from light emission:chemiluminescence was lower in brain microsomes obtained from CO group compared to the control group (without extract supplementation). Moreover, it was observed that the addition of the extract reduced chemiluminescence -measured as total cpm- in a concentration dependent manner. The fatty acid composition of brain microsomes from control group was profoundly modified when subjected to non-enzymatic lipoperoxidation with a considerable decrease of arachidonic acid C20:4ω6 and docosahexaenoic acid C22:6ω3. As a consequence, the unsaturation index, a parameter based on the maximal rate of oxidation of specific fatty acids, was higher in the CO group compared to controls. The simultaneous analysis of unsaturation index, chemiluminescence and fatty acid composition indicate that CO may act as an antioxidant protecting rat brain microsomes from peroxidative damage.
format Articulo
Articulo
author Palacios, Alejandro
Barberón, Javier
Leaden, Patricio
Zeinsteger, Pedro Adolfo
author_facet Palacios, Alejandro
Barberón, Javier
Leaden, Patricio
Zeinsteger, Pedro Adolfo
author_sort Palacios, Alejandro
title Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_short Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_full Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_fullStr Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_full_unstemmed Calendula officinalis L. (Asteraceae) possess antioxidant properties on Fe2+-initiated peroxidation of rat brain microsomes
title_sort calendula officinalis l. (asteraceae) possess antioxidant properties on fe2+-initiated peroxidation of rat brain microsomes
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/85835
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bdutipo_str Repositorios
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