Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner

Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fash...

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Autores principales: Errea, Agustina Juliana, Cayet, Delphine, Marchetti, Philippe, Tang, Cong, Kluza, Jerome, Offermanns, Stefan, Sirard, Jean-Claude, Rumbo, Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Médicas
Lactate
GPR81
Macrophage pro-inflammatory
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85969
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85969
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Lactate
GPR81
Macrophage pro-inflammatory
spellingShingle Ciencias Médicas
Lactate
GPR81
Macrophage pro-inflammatory
Errea, Agustina Juliana
Cayet, Delphine
Marchetti, Philippe
Tang, Cong
Kluza, Jerome
Offermanns, Stefan
Sirard, Jean-Claude
Rumbo, Martín
Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner
topic_facet Ciencias Médicas
Lactate
GPR81
Macrophage pro-inflammatory
description Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/-cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/-cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.
format Articulo
Articulo
author Errea, Agustina Juliana
Cayet, Delphine
Marchetti, Philippe
Tang, Cong
Kluza, Jerome
Offermanns, Stefan
Sirard, Jean-Claude
Rumbo, Martín
author_facet Errea, Agustina Juliana
Cayet, Delphine
Marchetti, Philippe
Tang, Cong
Kluza, Jerome
Offermanns, Stefan
Sirard, Jean-Claude
Rumbo, Martín
author_sort Errea, Agustina Juliana
title Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner
title_short Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner
title_full Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner
title_fullStr Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner
title_full_unstemmed Lactate inhibits the pro-inflammatory response and metabolic reprogramming in Murine macrophages in a GPR81-independent manner
title_sort lactate inhibits the pro-inflammatory response and metabolic reprogramming in murine macrophages in a gpr81-independent manner
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/85969
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bdutipo_str Repositorios
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