Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration

Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neuro...

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Detalles Bibliográficos
Autores principales: Rodrigo Albors, Aida, Tazaki, Akira, Rost, Fabian, Nowoshilow, Sergej, Chara, Osvaldo, Tanaka, Ely M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Exactas
Regeneration
Spinal cord
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86399
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-86399
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Regeneration
Spinal cord
spellingShingle Ciencias Exactas
Regeneration
Spinal cord
Rodrigo Albors, Aida
Tazaki, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Ely M.
Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
topic_facet Ciencias Exactas
Regeneration
Spinal cord
description Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
format Articulo
Articulo
author Rodrigo Albors, Aida
Tazaki, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Ely M.
author_facet Rodrigo Albors, Aida
Tazaki, Akira
Rost, Fabian
Nowoshilow, Sergej
Chara, Osvaldo
Tanaka, Ely M.
author_sort Rodrigo Albors, Aida
title Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_short Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_full Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_fullStr Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_full_unstemmed Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
title_sort planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/86399
work_keys_str_mv AT rodrigoalborsaida planarcellpolaritymediatedinductionofneuralstemcellexpansionduringaxolotlspinalcordregeneration
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AT rostfabian planarcellpolaritymediatedinductionofneuralstemcellexpansionduringaxolotlspinalcordregeneration
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