Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells

Tissue injury leads to the release of uric acid (UA). At high local concentrations, UA can form monosodium urate crystals (MSU). MSU and UA stimulate neutrophils to release extracellular traps (NET). Here, we investigated whether these NET could be involved in the development of inflammation by stim...

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Detalles Bibliográficos
Autores principales: Sabbione, Florencia, Keitelman, Irene A., Iula, Leonardo, Ferrero, Mariana, Giordano, Mirta N., Baldi, Pablo, Rumbo, Martín, Jancic, Carolina, Trevani, Analía Silvina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Bioquímica
Cigarette smoke
Human epithelial cells
Monosodium urate crystals
Neutrophil extracellular traps
Uric acid
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87471
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87471
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bioquímica
Cigarette smoke
Human epithelial cells
Monosodium urate crystals
Neutrophil extracellular traps
Uric acid
spellingShingle Ciencias Exactas
Bioquímica
Cigarette smoke
Human epithelial cells
Monosodium urate crystals
Neutrophil extracellular traps
Uric acid
Sabbione, Florencia
Keitelman, Irene A.
Iula, Leonardo
Ferrero, Mariana
Giordano, Mirta N.
Baldi, Pablo
Rumbo, Martín
Jancic, Carolina
Trevani, Analía Silvina
Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells
topic_facet Ciencias Exactas
Bioquímica
Cigarette smoke
Human epithelial cells
Monosodium urate crystals
Neutrophil extracellular traps
Uric acid
description Tissue injury leads to the release of uric acid (UA). At high local concentrations, UA can form monosodium urate crystals (MSU). MSU and UA stimulate neutrophils to release extracellular traps (NET). Here, we investigated whether these NET could be involved in the development of inflammation by stimulating cytokine release by airway epithelial cells. We found that NET significantly increased the secretion of CXCL8/IL-8 and IL-6 by alveolar and bronchial epithelial cells. These effects were not observed when NETosis was inhibited by Diphenyleneiodonium, elastase inhibitor, or Cl-amidine. Similar findings were made with NET induced by cigarette smoke extract, suggesting that NET proinflammatory capacity is independent of the inducing stimulus. Furthermore, NET affected neither the viability and morphology of epithelial cells nor the barrier integrity of polarized cells. The epithelial stimulatory capacity of NET was not affected by degradation of DNA with micrococcal nuclease, treatment with heparin, or inhibition of the elastase immobilized to DNA, but it was significantly reduced by pretreatment with an anti-HMGB-1 blocking antibody. Altogether, our findings indicate that NET exert direct proinflammatory effects on airway epithelial cells that might contribute in vivo to the further recruitment of neutrophils and the perpetuation of inflammation upon lung tissue damage.
format Articulo
Articulo
author Sabbione, Florencia
Keitelman, Irene A.
Iula, Leonardo
Ferrero, Mariana
Giordano, Mirta N.
Baldi, Pablo
Rumbo, Martín
Jancic, Carolina
Trevani, Analía Silvina
author_facet Sabbione, Florencia
Keitelman, Irene A.
Iula, Leonardo
Ferrero, Mariana
Giordano, Mirta N.
Baldi, Pablo
Rumbo, Martín
Jancic, Carolina
Trevani, Analía Silvina
author_sort Sabbione, Florencia
title Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells
title_short Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells
title_full Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells
title_fullStr Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells
title_full_unstemmed Neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells
title_sort neutrophil extracellular traps stimulate proinflammatory responses in human airway epithelial cells
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87471
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