Proinflammatory response of canine trophoblasts to <i>Brucella canis</i> infection

Brucella canis infection is an important cause of late-term abortion in pregnant bitches. The pathophysiological mechanisms leading to B. canis–induced abortion are unknown, but heavily infected trophoblasts are consistently observed. As trophoblasts responses to other pathogens contribute to placen...

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Autores principales: Fernández, Andrea G., Hielpos, M. Soledad, Ferrero, Mariana C., Fossati, Carlos Alberto, Baldi, Pablo C.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Brucella canis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87520
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87520
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Brucella canis
spellingShingle Ciencias Exactas
Brucella canis
Fernández, Andrea G.
Hielpos, M. Soledad
Ferrero, Mariana C.
Fossati, Carlos Alberto
Baldi, Pablo C.
Proinflammatory response of canine trophoblasts to <i>Brucella canis</i> infection
topic_facet Ciencias Exactas
Brucella canis
description Brucella canis infection is an important cause of late-term abortion in pregnant bitches. The pathophysiological mechanisms leading to B. canis–induced abortion are unknown, but heavily infected trophoblasts are consistently observed. As trophoblasts responses to other pathogens contribute to placental inflammation leading to abortion, the aim of the present study was to characterize the cytokine response of canine trophoblasts to B. canis infection. To achieve this, trophoblasts isolated from term placenta of healthy female dogs were infected with B. canis, culture supernatants were harvested for cytokine determinations, and the load of intracellular viable B. canis was determined at different times post-infection. Additionally, cytokine responses were assessed in non-infected trophoblasts stimulated with conditioned media (CM) from B. canis-infected canine monocytes and neutrophils. Finally, cytokine response and bacteria replication were assessed in canine placental explants infected ex vivo. B. canis successfully infected and replicated in primary canine trophoblasts, eliciting an increase in IL-8 and RANTES (CCL5) secretion. Moreover, the stimulation of trophoblasts with CM from B. canis-infected monocytes and neutrophils induced a significant increase in IL-8, IL-6 and RANTES secretion. B. canis replication was confirmed in infected placental explants and the infection elicited an increased secretion of TNF-α, IL-8, IL-6 and RANTES. This study shows that canine trophoblasts produce proinflammatory cytokines in response to B. canis infection and/or to stimulation with factors produced by infected monocytes and neutrophils. These cytokines may contribute to placental inflammation leading to abortion in B. canis-infected pregnant bitches.
format Articulo
Articulo
author Fernández, Andrea G.
Hielpos, M. Soledad
Ferrero, Mariana C.
Fossati, Carlos Alberto
Baldi, Pablo C.
author_facet Fernández, Andrea G.
Hielpos, M. Soledad
Ferrero, Mariana C.
Fossati, Carlos Alberto
Baldi, Pablo C.
author_sort Fernández, Andrea G.
title Proinflammatory response of canine trophoblasts to <i>Brucella canis</i> infection
title_short Proinflammatory response of canine trophoblasts to <i>Brucella canis</i> infection
title_full Proinflammatory response of canine trophoblasts to <i>Brucella canis</i> infection
title_fullStr Proinflammatory response of canine trophoblasts to <i>Brucella canis</i> infection
title_full_unstemmed Proinflammatory response of canine trophoblasts to <i>Brucella canis</i> infection
title_sort proinflammatory response of canine trophoblasts to <i>brucella canis</i> infection
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87520
work_keys_str_mv AT fernandezandreag proinflammatoryresponseofcaninetrophoblaststoibrucellacanisiinfection
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AT ferreromarianac proinflammatoryresponseofcaninetrophoblaststoibrucellacanisiinfection
AT fossaticarlosalberto proinflammatoryresponseofcaninetrophoblaststoibrucellacanisiinfection
AT baldipabloc proinflammatoryresponseofcaninetrophoblaststoibrucellacanisiinfection
bdutipo_str Repositorios
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