Circulating ghrelin acts on GABA neurons of the area postrema and mediates gastric emptying in male mice

Ghrelin is known to act on the area postrema (AP), a sensory circumventricular organ located in the medulla oblongata that regulates a variety of important physiological functions. However, the neuronal targets of ghrelin in the AP and their potential role are currently unknown. In this study, we us...

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Autores principales: Cabral, Agustina Soledad, Cornejo, María Paula, Fernández, Gimena, De Francesco, Pablo Nicolás, García Romero, Guadalupe, Uriarte Donati, Maia, Zigman, Jeffrey M., Portiansky, Enrique Leo, Reynaldo, Mirta Beatriz, Perelló, Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Biología
Ciencias Veterinarias
Ghrelin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87561
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87561
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Ciencias Veterinarias
Ghrelin
spellingShingle Ciencias Exactas
Biología
Ciencias Veterinarias
Ghrelin
Cabral, Agustina Soledad
Cornejo, María Paula
Fernández, Gimena
De Francesco, Pablo Nicolás
García Romero, Guadalupe
Uriarte Donati, Maia
Zigman, Jeffrey M.
Portiansky, Enrique Leo
Reynaldo, Mirta Beatriz
Perelló, Mario
Circulating ghrelin acts on GABA neurons of the area postrema and mediates gastric emptying in male mice
topic_facet Ciencias Exactas
Biología
Ciencias Veterinarias
Ghrelin
description Ghrelin is known to act on the area postrema (AP), a sensory circumventricular organ located in the medulla oblongata that regulates a variety of important physiological functions. However, the neuronal targets of ghrelin in the AP and their potential role are currently unknown. In this study, we used wild-type and genetically modified mice to gain insights into the neurons of the AP expressing the ghrelin receptor [growth hormone secretagogue receptor (GHSR)] and their role. We show that circulating ghrelin mainly accesses the AP but not to the adjacent nucleus of the solitary tract. Also, we show that both peripheral administration of ghrelin and fasting induce an increase of c-Fos, a marker of neuronal activation, in GHSR-expressing neurons of the AP, and that GHSR expression is necessary for the fasting-induced activation of AP neurons. Additionally, we show that ghrelin-sensitive neurons of the AP are mainly γ-aminobutyric acid (GABA)ergic, and that an intact AP is required for ghrelin-induced gastric emptying. Overall, we show that the capacity of circulating ghrelin to acutely induce gastric emptying in mice requires the integrity of the AP, which contains a population of GABA neurons that are a target of plasma ghrelin.
format Articulo
Articulo
author Cabral, Agustina Soledad
Cornejo, María Paula
Fernández, Gimena
De Francesco, Pablo Nicolás
García Romero, Guadalupe
Uriarte Donati, Maia
Zigman, Jeffrey M.
Portiansky, Enrique Leo
Reynaldo, Mirta Beatriz
Perelló, Mario
author_facet Cabral, Agustina Soledad
Cornejo, María Paula
Fernández, Gimena
De Francesco, Pablo Nicolás
García Romero, Guadalupe
Uriarte Donati, Maia
Zigman, Jeffrey M.
Portiansky, Enrique Leo
Reynaldo, Mirta Beatriz
Perelló, Mario
author_sort Cabral, Agustina Soledad
title Circulating ghrelin acts on GABA neurons of the area postrema and mediates gastric emptying in male mice
title_short Circulating ghrelin acts on GABA neurons of the area postrema and mediates gastric emptying in male mice
title_full Circulating ghrelin acts on GABA neurons of the area postrema and mediates gastric emptying in male mice
title_fullStr Circulating ghrelin acts on GABA neurons of the area postrema and mediates gastric emptying in male mice
title_full_unstemmed Circulating ghrelin acts on GABA neurons of the area postrema and mediates gastric emptying in male mice
title_sort circulating ghrelin acts on gaba neurons of the area postrema and mediates gastric emptying in male mice
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87561
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