Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure

Although non-innervated, the placenta contains both cholinesterases (ChEs), butyryl-cholinesterase (BChE) and acetylcholinesterase (AChE). These enzymes are well-known fortheir multiple molecular forms. In a first approach, we used recognized specific inhibitors,substrate preferences and non-denatur...

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Autores principales: Sanchez, Susana, Vera, Berta, Montagna, Cristina, Magnarelli, Gladis
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: Elsevier 2014
Materias:
Placenta
Butyrylcholinesterase
Acetylcholinesterase
Organophosphates
Ciencias Biomédicas
Acceso en línea:http://rdi.uncoma.edu.ar/handle/uncomaid/15221
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-15221
record_format dspace
spelling I22-R178-uncomaid-152212023-08-31T14:32:03Z Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure Sanchez, Susana Vera, Berta Montagna, Cristina Magnarelli, Gladis Placenta Butyrylcholinesterase Acetylcholinesterase Organophosphates Ciencias Biomédicas Although non-innervated, the placenta contains both cholinesterases (ChEs), butyryl-cholinesterase (BChE) and acetylcholinesterase (AChE). These enzymes are well-known fortheir multiple molecular forms. In a first approach, we used recognized specific inhibitors,substrate preferences and non-denaturating gel electrophoresis in order to characterize theChE profile of term placenta from uncomplicated pregnancy. Results strongly suggest thatthe predominant cholinesterasic form present was tetrameric BChE.It is well established that both ChEs are targets of cholinesterase-inhibiting organophos-phates (OP), one of the most important classes of chemicals actively applied to theenvironment. However, we have previously reported increased ChEs activity in placenta ofrural residents exposed to OP. In the present work, we have studied: 1) whether this find-ing was reproducible and, 2) whether AChE or BChE up regulation is behind the increase ofplacental ChE activity. The population studied included forty healthy women who live inan agricultural area. Samples were collected during both the OP pulverization period (PP)and the recess period (RP). The placental ChEs activity increased in PP, evidencing repro-ducibility of previous results. The analysis of non-denaturating gels revealed that increasedactivity of total ChE activity in placenta from women exposed to OP may be attributable totetrameric BChE up-regulation. Fil: Sanchez, Susana. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. Fil: Vera, Berta. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Fil: Vera, Berta. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. Fil: Montagna, Cristina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Fil: Montagna, Cristina. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y Salud; Argentina. Fil: Magnarelli, Gladis. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Fil: Magnarelli, Gladis. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. 2014-11 2019-05-30T14:05:43Z 2019-05-30T14:05:43Z Articulo article acceptedVersion http://rdi.uncoma.edu.ar/handle/uncomaid/15221 2214-7500 eng https://www.journals.elsevier.com/toxicology-reports Atribución-NoComercial-SinDerivadas 2.5 Argentina https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ application/pdf pp. 437–442 application/pdf Elsevier Board Toxicology Reports Vol. 2 (2015)
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Placenta
Butyrylcholinesterase
Acetylcholinesterase
Organophosphates
Ciencias Biomédicas
spellingShingle Placenta
Butyrylcholinesterase
Acetylcholinesterase
Organophosphates
Ciencias Biomédicas
Sanchez, Susana
Vera, Berta
Montagna, Cristina
Magnarelli, Gladis
Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure
topic_facet Placenta
Butyrylcholinesterase
Acetylcholinesterase
Organophosphates
Ciencias Biomédicas
description Although non-innervated, the placenta contains both cholinesterases (ChEs), butyryl-cholinesterase (BChE) and acetylcholinesterase (AChE). These enzymes are well-known fortheir multiple molecular forms. In a first approach, we used recognized specific inhibitors,substrate preferences and non-denaturating gel electrophoresis in order to characterize theChE profile of term placenta from uncomplicated pregnancy. Results strongly suggest thatthe predominant cholinesterasic form present was tetrameric BChE.It is well established that both ChEs are targets of cholinesterase-inhibiting organophos-phates (OP), one of the most important classes of chemicals actively applied to theenvironment. However, we have previously reported increased ChEs activity in placenta ofrural residents exposed to OP. In the present work, we have studied: 1) whether this find-ing was reproducible and, 2) whether AChE or BChE up regulation is behind the increase ofplacental ChE activity. The population studied included forty healthy women who live inan agricultural area. Samples were collected during both the OP pulverization period (PP)and the recess period (RP). The placental ChEs activity increased in PP, evidencing repro-ducibility of previous results. The analysis of non-denaturating gels revealed that increasedactivity of total ChE activity in placenta from women exposed to OP may be attributable totetrameric BChE up-regulation.
format Articulo
article
acceptedVersion
author Sanchez, Susana
Vera, Berta
Montagna, Cristina
Magnarelli, Gladis
author_facet Sanchez, Susana
Vera, Berta
Montagna, Cristina
Magnarelli, Gladis
author_sort Sanchez, Susana
title Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure
title_short Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure
title_full Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure
title_fullStr Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure
title_full_unstemmed Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure
title_sort characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure
publisher Elsevier
publishDate 2014
url http://rdi.uncoma.edu.ar/handle/uncomaid/15221
work_keys_str_mv AT sanchezsusana characterizationofplacentalcholinesterasesandactivityinductionassociatedtoenvironmentalorganophosphateexposure
AT veraberta characterizationofplacentalcholinesterasesandactivityinductionassociatedtoenvironmentalorganophosphateexposure
AT montagnacristina characterizationofplacentalcholinesterasesandactivityinductionassociatedtoenvironmentalorganophosphateexposure
AT magnarelligladis characterizationofplacentalcholinesterasesandactivityinductionassociatedtoenvironmentalorganophosphateexposure
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