Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage

Chlorpyrifos (CPF) is an insecticide widely used for pest control in the fruit-productive region of North Patagonia, Argentina, where it is found in superficial waters. The aim of this study was to establish the toxic effects of CPF in Rhinella arenarum toad larvae as a potentially exposed species....

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Autores principales: Liendro, Natacha, Ferrari, Ana, Mardirosian, Mariana, Lascano, Cecilia Inés, Venturino, Andrés
Formato: Articulo article acceptedVersion
Lenguaje:Inglés
Publicado: Elsevier Science 2015
Materias:
Amphibians
Organophosphates
Biomarkers
Cholinesterase inhibition
Oxidative stress
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
Acceso en línea:http://rdi.uncoma.edu.ar/handle/uncomaid/17058
Aporte de:
Repositorio Institucional UNCo de Universidad Nacional del Comahue
id I22-R178-uncomaid-17058
record_format dspace
spelling I22-R178-uncomaid-170582023-10-04T13:56:41Z Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage Liendro, Natacha Ferrari, Ana Mardirosian, Mariana Lascano, Cecilia Inés Venturino, Andrés Amphibians Organophosphates Biomarkers Cholinesterase inhibition Oxidative stress Ciencias de la Tierra y Medio Ambiente Ciencias Agrarias y Forestales Chlorpyrifos (CPF) is an insecticide widely used for pest control in the fruit-productive region of North Patagonia, Argentina, where it is found in superficial waters. The aim of this study was to establish the toxic effects of CPF in Rhinella arenarum toad larvae as a potentially exposed species. We determined the 96 h-LC50 (1.46 ± 0.27 mg/L), the LOEC (0.81 mg/L, LC10) and NOEC (0.43 mg/L, LC1) for CPF lethality as endpoint. We also analyzed biochemical biomarkers in larvae exposed to sublethal CPF concentrations. The IC50 for cholinesterase was 0.113 ± 0.026 mg/L, one order of magnitude lower than the LC50. Carboxylesterase activity was inhibited, buffering OP toxicity on cholinesterase. Reduced glutathione increased after 24 h as an antioxidant response, and decreased at 96 h together with catalase activity, due to oxidative stress. These biochemical effects suggest that environmentally relevant CPF concentrations pose a threat to R. arenarum larvae progression. Fil: Liendro, Natacha. Universidad Nacional del Comahue. Facultad de Ingeniería. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina. Fil: Liendro, Natacha. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Liendro, Natacha. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Ferrari, Ana. Universidad Nacional del Comahue. Facultad de Ingeniería. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina. Fil: Ferrari, Ana. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. Fil: Ferrari, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Mardirosian, Mariana Noelia. Universidad Nacional del Comahue. Facultad de Ingeniería. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina. Fil: Mardirosian, Mariana Noelia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Mardirosian, Mariana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Lascano, Cecilia Ines. Universidad Nacional del Comahue. Facultad de Ingeniería. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina. Fil: Lascano, Cecilia Ines. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Lascano, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Venturino, Andrés. Universidad Nacional del Comahue. Facultad de Ingeniería. Laboratorio de Investigaciones Bioquímicas y Químicas del Ambiente; Argentina. Fil: Venturino, Andrés. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Venturino, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2015-03 2023-03-27T12:57:03Z 2023-03-27T12:57:03Z Articulo article acceptedVersion 1382-6689 http://rdi.uncoma.edu.ar/handle/uncomaid/17058 eng http://hdl.handle.net/11336/37575 Atribución-NoComercial-CompartirIgual 2.5 Argentina https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ application/pdf pp. 525-535 application/pdf Elsevier Science Environmental Toxicology and Pharmacology. Núm. 2 Vol. 39 (2015)
institution Universidad Nacional del Comahue
institution_str I-22
repository_str R-178
collection Repositorio Institucional UNCo
language Inglés
topic Amphibians
Organophosphates
Biomarkers
Cholinesterase inhibition
Oxidative stress
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
spellingShingle Amphibians
Organophosphates
Biomarkers
Cholinesterase inhibition
Oxidative stress
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
Liendro, Natacha
Ferrari, Ana
Mardirosian, Mariana
Lascano, Cecilia Inés
Venturino, Andrés
Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage
topic_facet Amphibians
Organophosphates
Biomarkers
Cholinesterase inhibition
Oxidative stress
Ciencias de la Tierra y Medio Ambiente
Ciencias Agrarias y Forestales
description Chlorpyrifos (CPF) is an insecticide widely used for pest control in the fruit-productive region of North Patagonia, Argentina, where it is found in superficial waters. The aim of this study was to establish the toxic effects of CPF in Rhinella arenarum toad larvae as a potentially exposed species. We determined the 96 h-LC50 (1.46 ± 0.27 mg/L), the LOEC (0.81 mg/L, LC10) and NOEC (0.43 mg/L, LC1) for CPF lethality as endpoint. We also analyzed biochemical biomarkers in larvae exposed to sublethal CPF concentrations. The IC50 for cholinesterase was 0.113 ± 0.026 mg/L, one order of magnitude lower than the LC50. Carboxylesterase activity was inhibited, buffering OP toxicity on cholinesterase. Reduced glutathione increased after 24 h as an antioxidant response, and decreased at 96 h together with catalase activity, due to oxidative stress. These biochemical effects suggest that environmentally relevant CPF concentrations pose a threat to R. arenarum larvae progression.
format Articulo
article
acceptedVersion
author Liendro, Natacha
Ferrari, Ana
Mardirosian, Mariana
Lascano, Cecilia Inés
Venturino, Andrés
author_facet Liendro, Natacha
Ferrari, Ana
Mardirosian, Mariana
Lascano, Cecilia Inés
Venturino, Andrés
author_sort Liendro, Natacha
title Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage
title_short Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage
title_full Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage
title_fullStr Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage
title_full_unstemmed Toxicity of the insecticide chlorpyrifos to the South American toad Rhinella arenarum at larval developmental stage
title_sort toxicity of the insecticide chlorpyrifos to the south american toad rhinella arenarum at larval developmental stage
publisher Elsevier Science
publishDate 2015
url http://rdi.uncoma.edu.ar/handle/uncomaid/17058
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AT mardirosianmariana toxicityoftheinsecticidechlorpyrifostothesouthamericantoadrhinellaarenarumatlarvaldevelopmentalstage
AT lascanoceciliaines toxicityoftheinsecticidechlorpyrifostothesouthamericantoadrhinellaarenarumatlarvaldevelopmentalstage
AT venturinoandres toxicityoftheinsecticidechlorpyrifostothesouthamericantoadrhinellaarenarumatlarvaldevelopmentalstage
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