Susceptibility of placental mitochondria to oxidative stress
Background: Two different mitochondrial fractions (MFs) have been character- ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification...
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2018
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| Acceso en línea: | https://rdi.uncoma.edu.ar/handle/uncomaid/19298 |
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I22-R178-uncomaid-192982026-04-24T17:13:27Z Susceptibility of placental mitochondria to oxidative stress Papa Gobbi, Rodrigo Magnarelli, Gladis Rovedatti, María Gabriela Mitochondria Placenta Oxidative stress Reactive oxygen species Ciencias de la Tierra y Medio Ambiente Ciencias Biomédicas Artículos Background: Two different mitochondrial fractions (MFs) have been character- ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a seri- ous threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and suscepti- bility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in pla- cental HMF and LMF performed in a cell-free assay. The small number of antioxi- dant system parameters measured did not allow detecting differences between HMF and LMF. Fil: Papa Gobbi, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Papa Gobbi, Rodrigo. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Papa Gobbi, Rodrigo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina. Fil: Magnarelli, Gladis Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina Fil: Rovedatti, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Rovedatti, María Gabriela. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. Fil: Rovedatti, María Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. 2018 2026-04-13T14:34:01Z 2026-04-13T14:34:01Z Articulo article acceptedVersion 2472-1727 https://rdi.uncoma.edu.ar/handle/uncomaid/19298 eng DOI: 10.1002/bdr2.1377 https://ri.conicet.gov.ar/handle/11336/212681 Atribución-NoComercial-CompartirIgual 4.0 https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf pp. 1228-1232 application/pdf John Wiley & Sons Birth Defects Research. 2018, 110 |
| institution |
Universidad Nacional del Comahue |
| institution_str |
I-22 |
| repository_str |
R-178 |
| collection |
Repositorio Institucional UNCo |
| language |
Inglés |
| topic |
Mitochondria Placenta Oxidative stress Reactive oxygen species Ciencias de la Tierra y Medio Ambiente Ciencias Biomédicas Artículos |
| spellingShingle |
Mitochondria Placenta Oxidative stress Reactive oxygen species Ciencias de la Tierra y Medio Ambiente Ciencias Biomédicas Artículos Papa Gobbi, Rodrigo Magnarelli, Gladis Rovedatti, María Gabriela Susceptibility of placental mitochondria to oxidative stress |
| topic_facet |
Mitochondria Placenta Oxidative stress Reactive oxygen species Ciencias de la Tierra y Medio Ambiente Ciencias Biomédicas Artículos |
| description |
Background: Two different mitochondrial fractions (MFs) have been character-
ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF).
Although these organelles are the main source of reactive oxygen species, an
imbalance between their production and the rate of detoxification represents a seri-
ous threat to mitochondrial homeostasis and, in the case of the placenta, also to the
fetus. The aim of this study was to evaluate the antioxidant capacity and suscepti-
bility to oxidative stress in both types of MFs.
Methods: Human MFs were isolated from healthy donors (n = 11) and either
incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione
(GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were
determined.
Results: H2O2 treatment increased LP and PC levels and decreased CAT activity.
GSH levels were similar in control and treated MFs.
Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the
antioxidant system measured in these two MFs responded similarly. To the best of
our knowledge, this is the first partial description of the antioxidant defense in pla-
cental HMF and LMF performed in a cell-free assay. The small number of antioxi-
dant system parameters measured did not allow detecting differences between
HMF and LMF. |
| format |
Articulo article acceptedVersion |
| author |
Papa Gobbi, Rodrigo Magnarelli, Gladis Rovedatti, María Gabriela |
| author_facet |
Papa Gobbi, Rodrigo Magnarelli, Gladis Rovedatti, María Gabriela |
| author_sort |
Papa Gobbi, Rodrigo |
| title |
Susceptibility of placental mitochondria to oxidative stress |
| title_short |
Susceptibility of placental mitochondria to oxidative stress |
| title_full |
Susceptibility of placental mitochondria to oxidative stress |
| title_fullStr |
Susceptibility of placental mitochondria to oxidative stress |
| title_full_unstemmed |
Susceptibility of placental mitochondria to oxidative stress |
| title_sort |
susceptibility of placental mitochondria to oxidative stress |
| publisher |
John Wiley & Sons |
| publishDate |
2018 |
| url |
https://rdi.uncoma.edu.ar/handle/uncomaid/19298 |
| work_keys_str_mv |
AT papagobbirodrigo susceptibilityofplacentalmitochondriatooxidativestress AT magnarelligladis susceptibilityofplacentalmitochondriatooxidativestress AT rovedattimariagabriela susceptibilityofplacentalmitochondriatooxidativestress |
| _version_ |
1865591717195939840 |