Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano

The S-layers (surface layer proteins) play a role in the probiotic properties of the strains that influenciate the immune response and in many cases promoting cell adhesion. Purified S-layer protein of Lactobacillus acidophilus ATCC 4356 has antimicrobial and antiviral properties that could be explo...

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Autor principal: García Díaz, Génesis Daniela
Otros Autores: Prado Acosta, Mariano
Formato: Tesis de maestría acceptedVersion
Lenguaje:Español
Publicado: Facultad de Farmacia y Bioquímica 2017
Materias:
S-Layers
Surface layer
Propiedades probióticos
Lactobacillus acidophilus
Agentes patógenos
Ciencia de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_1541
http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_1541.dir/1541.PDF
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
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record_format dspace
spelling I28-R145-HWA_15412019-09-27 The S-layers (surface layer proteins) play a role in the probiotic properties of the strains that influenciate the immune response and in many cases promoting cell adhesion. Purified S-layer protein of Lactobacillus acidophilus ATCC 4356 has antimicrobial and antiviral properties that could be exploited. With a similar objective will seek for these properties in the S-layer proteins of strains of Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri, against different pathogens. We studied how this bacterial surface structure can influence in the probiotic characteristics such as binding to epithelial cells, and the barrier effect against pathogens (bacterias and virus). Through construction of Pseudomonas aeruginosa strains with fluorescent reporter gene we verified the ability of the purified S-layer protein to protect eukaryotic cell cultures against bacterial and viral pathogens and their effect on biofilm formation on biotic and abiotic surfaces.\nA considerable decrease in biofilm formation of P. aeruginosa was obtained when this was treated with S-layer of L. acidophilus, L. brevis and L. helveticus. Also we found a decrease in the adhesion of the pathogen to A549 cells pretreated with S-layer proteins. A decreased was observed for viral infection of HSV-1 to A549 cells pretreated with S-layers proteins studied, except that of L. kefiri. Fil: García Díaz, Génesis Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Buenos Aires, Argentina Prado Acosta, Mariano Facultad de Farmacia y Bioquímica Ruzal, Sandra M. García Díaz, Génesis Daniela 2017-12-20 Las S-layers (surface layer) o capa S cumplen un rol en las propiedades probióticas de las cepas que la poseen siendo capaz de influir en la respuesta inmune y en muchos casos favoreciendo la adhesión a células.\nLa proteína S-layer pura de Lactobacillus acidophilus ATCC 4356 tiene propiedades antimicrobianas y antivirales que podrían ser explotadas. Con un objetivo similar se buscará verificarlo en las cepas de Lactobacillus brevis, Lactobacillus helveticus y Lactobacillus kefiri, frente a diferentes patógenos.\nSe buscó determinar cómo puede influir esta estructura de la superficie bacteriana en las características probióticas como son capacidad de adhesión a células epiteliales, y el efecto barrera contra patógenos (bacterianos y virales).\nA través de construcciones de cepas de Pseudomonas aeruginosa con genes reporteros fluorescentes se verificó la capacidad de la proteína S-layer pura de proteger a cultivos de células eucariotas frente a agentes patógenos bacterianos y virales y su efecto sobre la formación de biofilm en superficies bióticas y abióticas.\nHemos obtenido una disminución considerable en la formación de biofilm de P. aeruginosa al tratarla con S-layer de L. acidophilus, L. brevis y L. helveticus. Además se encontró una disminución en la adhesión del patógeno a células A549 en células pretratadas con las proteínas. También se observó disminución de la infección viral de HSV-1 al tratar previamente células A549 con las proteínas S- layer estudiadas a excepción de la S-layer de L. acidophilus. application/pdf Cavallaro, Lucía Flichman, Diego Romanowski, Víctor S-Layers Surface layer Propiedades probióticos Lactobacillus acidophilus Agentes patógenos spa Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Ciencia de la vida Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano info:eu-repo/semantics/masterThesis info:ar-repo/semantics/tesis de maestría info:eu-repo/semantics/acceptedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_1541 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_1541.dir/1541.PDF
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
language Español
orig_language_str_mv spa
topic S-Layers
Surface layer
Propiedades probióticos
Lactobacillus acidophilus
Agentes patógenos
Ciencia de la vida
spellingShingle S-Layers
Surface layer
Propiedades probióticos
Lactobacillus acidophilus
Agentes patógenos
Ciencia de la vida
García Díaz, Génesis Daniela
Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano
topic_facet S-Layers
Surface layer
Propiedades probióticos
Lactobacillus acidophilus
Agentes patógenos
Ciencia de la vida
description The S-layers (surface layer proteins) play a role in the probiotic properties of the strains that influenciate the immune response and in many cases promoting cell adhesion. Purified S-layer protein of Lactobacillus acidophilus ATCC 4356 has antimicrobial and antiviral properties that could be exploited. With a similar objective will seek for these properties in the S-layer proteins of strains of Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri, against different pathogens. We studied how this bacterial surface structure can influence in the probiotic characteristics such as binding to epithelial cells, and the barrier effect against pathogens (bacterias and virus). Through construction of Pseudomonas aeruginosa strains with fluorescent reporter gene we verified the ability of the purified S-layer protein to protect eukaryotic cell cultures against bacterial and viral pathogens and their effect on biofilm formation on biotic and abiotic surfaces.\nA considerable decrease in biofilm formation of P. aeruginosa was obtained when this was treated with S-layer of L. acidophilus, L. brevis and L. helveticus. Also we found a decrease in the adhesion of the pathogen to A549 cells pretreated with S-layer proteins. A decreased was observed for viral infection of HSV-1 to A549 cells pretreated with S-layers proteins studied, except that of L. kefiri.
author2 Prado Acosta, Mariano
author_facet Prado Acosta, Mariano
García Díaz, Génesis Daniela
format Tesis de maestría
Tesis de maestría
acceptedVersion
author García Díaz, Génesis Daniela
author_sort García Díaz, Génesis Daniela
title Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano
title_short Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano
title_full Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano
title_fullStr Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano
title_full_unstemmed Propiedades de la proteína de capa S (S-layer) de cepas probióticas de Lactobacillus : efecto antiviral y antibacteriano
title_sort propiedades de la proteína de capa s (s-layer) de cepas probióticas de lactobacillus : efecto antiviral y antibacteriano
publisher Facultad de Farmacia y Bioquímica
publishDate 2017
url http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_1541
http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_1541.dir/1541.PDF
work_keys_str_mv AT garciadiazgenesisdaniela propiedadesdelaproteinadecapasslayerdecepasprobioticasdelactobacillusefectoantiviralyantibacteriano
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