InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de...
Cryopreservation of gastrointestinal nematode infective larvae (L3) has been used to preserver gastrointestinal nematodes in laboratory for a long time. The aim of this study was to establish the survival and infective capability of infective larvae after cryopreservation. A total of 2 isolates name...
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| Autores principales: | , , , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2012
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3785 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3785.dir/3785.PDF |
| Aporte de: |
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I28-R145-HWA_3785 |
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| record_format |
dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| language |
Español |
| orig_language_str_mv |
spa |
| topic |
Criopreservación Haemonchus Ovinos Descongelado Infección Criopreservation Haemonchus Sheep Thawing Infection |
| spellingShingle |
Criopreservación Haemonchus Ovinos Descongelado Infección Criopreservation Haemonchus Sheep Thawing Infection Guzmán, M. Fiel, C.A. Steffan, P.E. Riva, E. Bernat, G. InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de... |
| topic_facet |
Criopreservación Haemonchus Ovinos Descongelado Infección Criopreservation Haemonchus Sheep Thawing Infection |
| description |
Cryopreservation of gastrointestinal nematode infective larvae (L3) has been used to preserver gastrointestinal nematodes in laboratory for a long time. The aim of this study was to establish the survival and infective capability of infective larvae after cryopreservation. A total of 2 isolates named as Ayacucho and Balcarce were used and L3 were exsheathed and thereafter, frozen in liquid nitrogen. Rate of survival (%) was estimated taking into account larval motility after thawing as discriminate factor for live/dead at 3 and 7 months from freezing. Six lambs were infected orally with L3 previously cryopreserved. The nematode infection was monitored through egg excretion in faeces. The rate of survival was 31-70% at 3 months and 68-72% at 7 months of freezing. Nematode eggs in faecessamples were detected at 3 weeks after infection being recorded no differences among isolates and/or freezing period of time. Advantages of the cryopreservation in nematodes research are emphasizing. |
| format |
Artículo Artículo publishedVersion |
| author |
Guzmán, M. Fiel, C.A. Steffan, P.E. Riva, E. Bernat, G. |
| author_facet |
Guzmán, M. Fiel, C.A. Steffan, P.E. Riva, E. Bernat, G. |
| author_sort |
Guzmán, M. |
| title |
InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de... |
| title_short |
InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de... |
| title_full |
InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de... |
| title_fullStr |
InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de... |
| title_full_unstemmed |
InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de... |
| title_sort |
invet. 2012, 14(1): 25-31 issn 1514-6634 (impreso) criopreservación de... |
| publisher |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. |
| publishDate |
2012 |
| url |
http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3785 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3785.dir/3785.PDF |
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1824359794023071744 |
| spelling |
I28-R145-HWA_37852024-11-05 InVet. 2012, 14(1): 25-31 ISSN 1514-6634 (impreso) CriopreservaCión de... Cryopreservation of gastrointestinal nematode infective larvae (L3) has been used to preserver gastrointestinal nematodes in laboratory for a long time. The aim of this study was to establish the survival and infective capability of infective larvae after cryopreservation. A total of 2 isolates named as Ayacucho and Balcarce were used and L3 were exsheathed and thereafter, frozen in liquid nitrogen. Rate of survival (%) was estimated taking into account larval motility after thawing as discriminate factor for live/dead at 3 and 7 months from freezing. Six lambs were infected orally with L3 previously cryopreserved. The nematode infection was monitored through egg excretion in faeces. The rate of survival was 31-70% at 3 months and 68-72% at 7 months of freezing. Nematode eggs in faecessamples were detected at 3 weeks after infection being recorded no differences among isolates and/or freezing period of time. Advantages of the cryopreservation in nematodes research are emphasizing. Fil: Guzmán, M. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva (SAMP). Área de Parasitología. Tandil, Argentina Fil: Guzmán, M. CONICET. Argentina Fil: Fiel, C.A. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva (SAMP). Área de Parasitología. Tandil, Argentina Fil: Steffan, P.E. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva (SAMP). Área de Parasitología. Tandil, Argentina Fil: Riva, E. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva (SAMP). Área de Parasitología. Tandil, Argentina Fil: Riva, E. CONICET. Argentina Fil: Bernat, G. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva (SAMP). Área de Parasitología. Tandil, Argentina Fil: Bernat, G. Comisión de Investigaciones Científicas (CIC). Buenos Aires, Argentina Guzmán, M. Fiel, C.A. Steffan, P.E. Riva, E. Bernat, G. 2012 La criopreservación de larvas infectantes (L3) ha permitido mantener nematodes gastrointestinales en laboratorio por prolongados periodos. El objetivo de este trabajo fue determinar la tasa de supervivencia y la capacidad infectiva post-descongelado de L3 de nematodes gastrointestinales. Para ello se utilizaron L3 denominadas Ayacucho y Balcarce, que fueron desenvainadas y posteriormente congeladas en nitrógeno líquido. Transcurrido un período de 3 y 7 meses, las larvas fueron descongeladas calculándose la tasa de supervivencia (%) considerando la movilidad como vital. La capacidad infectiva se determinó mediante la excreción de huevos en materia fecal de ovinos infectados con L3 criopreservadas. La supervivencia post-descongelado fue 31-70% a los 3 meses y 68-72% a los 7 meses. Los animales comenzaron a eliminar huevos a los 21 días post-inoculación, independientemente del tiempo de congelado y del origen de las larvas. Se destaca la contribución significativa a la investigación en parasitología veterinaria de la técnica de criopreservación. application/pdf 1514-6634 (impreso) 1668-3498 (en l?nea) Criopreservación Haemonchus Ovinos Descongelado Infección Criopreservation Haemonchus Sheep Thawing Infection spa Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ InVet, vol. 14, nº1 Supervivencia y capacidad infectiva de larvas criopreservadas de nematodes trichostrongylideos Survival and infective capability of cryopreserved infective larvae of trichostongyles info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3785 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3785.dir/3785.PDF |