δ-aminolaevulinate synthetase in extracts of cultured soybean cells

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1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was presen...

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Autores principales:	De Xifra, E.A.W., Del C. Batlle, A.M., Tigier, H.A.
Formato:	Artículo publishedVersion
Publicado:	1971
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00052744_v235_n3_p511_DeXifra https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00052744_v235_n3_p511_DeXifra_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

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spelling	I28-R145-paper_00052744_v235_n3_p511_DeXifra_oai2024-08-16 De Xifra, E.A.W. Del C. Batlle, A.M. Tigier, H.A. 1971 1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was present in the soluble fraction and was very labile. 3. 3. When crude extracts or 500 × g supernatant were stored at 4-6°, the apparent activity of δ-aminolaevulinate synthetase increased by as much as 3-6 times, while the activities of δ-aminolaevulinate dehydratase and succinyl-CoA synthetase did not significantly change during the storage. Activation was dependent on concentrations of cells suspensions during disruption and aging. 4. 4. Gel filtration with Sephadex G-25 of 2000 × g supernatants produced an enzyme fraction 30% more active. An increase in enzyme activity was observed when dark-grown callus were exposed to light. 5. 5. The addition of ATP, gibberellic acid and δ-aminolaevulinate to the culture media diminished activity; iron deficiency also produced an δ-aminolaevulinate synthetase less active. © 1971. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tigier, H.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00052744_v235_n3_p511_DeXifra info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar BBA - Enzymology 1971;235(3):511-517 δ-aminolaevulinate synthetase in extracts of cultured soybean cells info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00052744_v235_n3_p511_DeXifra_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
description	1. 1. δ-Aminolaevulinate synthetase has been detected in extracts of soybean callus tissues and the enzyme activity reached its maximum when callus were 11 days old. 2. 2. The presence of a compound which seems to control δ-aminolaevulinate synthetase activity was demonstrated. The enzyme was present in the soluble fraction and was very labile. 3. 3. When crude extracts or 500 × g supernatant were stored at 4-6°, the apparent activity of δ-aminolaevulinate synthetase increased by as much as 3-6 times, while the activities of δ-aminolaevulinate dehydratase and succinyl-CoA synthetase did not significantly change during the storage. Activation was dependent on concentrations of cells suspensions during disruption and aging. 4. 4. Gel filtration with Sephadex G-25 of 2000 × g supernatants produced an enzyme fraction 30% more active. An increase in enzyme activity was observed when dark-grown callus were exposed to light. 5. 5. The addition of ATP, gibberellic acid and δ-aminolaevulinate to the culture media diminished activity; iron deficiency also produced an δ-aminolaevulinate synthetase less active. © 1971.
format	Artículo Artículo publishedVersion
author	De Xifra, E.A.W. Del C. Batlle, A.M. Tigier, H.A.
spellingShingle	De Xifra, E.A.W. Del C. Batlle, A.M. Tigier, H.A. δ-aminolaevulinate synthetase in extracts of cultured soybean cells
author_facet	De Xifra, E.A.W. Del C. Batlle, A.M. Tigier, H.A.
author_sort	De Xifra, E.A.W.
title	δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_short	δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_full	δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_fullStr	δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_full_unstemmed	δ-aminolaevulinate synthetase in extracts of cultured soybean cells
title_sort	δ-aminolaevulinate synthetase in extracts of cultured soybean cells
publishDate	1971
url	http://hdl.handle.net/20.500.12110/paper_00052744_v235_n3_p511_DeXifra https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00052744_v235_n3_p511_DeXifra_oai
work_keys_str_mv	AT dexifraeaw daminolaevulinatesynthetaseinextractsofculturedsoybeancells AT delcbatlleam daminolaevulinatesynthetaseinextractsofculturedsoybeancells AT tigierha daminolaevulinatesynthetaseinextractsofculturedsoybeancells
_version_	1809356859572748288

δ-aminolaevulinate synthetase in extracts of cultured soybean cells

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