Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α
Prostaglandin F2α (PGF2α) induces cyclin D1 expression and DNA synthesis in Swiss 3T3 cells. In order to assess which signaling mechanisms are implicated in these processes, we have used both a pharmacological approach and interfering mutants. We demonstrate that PGF2α induces extracellular-signal-r...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00145793_v580_n10_p2512_Dekanty https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v580_n10_p2512_Dekanty_oai |
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I28-R145-paper_00145793_v580_n10_p2512_Dekanty_oai2024-08-16 Dekanty, A. Giulianelli, S. Coso, O.A. Rudland, P.S. Jimenez de Asua, L. 2006 Prostaglandin F2α (PGF2α) induces cyclin D1 expression and DNA synthesis in Swiss 3T3 cells. In order to assess which signaling mechanisms are implicated in these processes, we have used both a pharmacological approach and interfering mutants. We demonstrate that PGF2α induces extracellular-signal-regulated kinase (ERK1-2) and p38MAPK activation, and inhibition of any of these signaling pathways completely blocks PGF2α-stimulated DNA synthesis. We also show that ERK1-2, but not p38MAPK activation is required to induce cyclin D1 expression, strongly suggesting that the concerted action of cyclin D1 gene expression and other events are required to induce complete phosphorylation of retinoblastoma protein and S-phase entry in response to PGF2α. © 2006. Fil:Dekanty, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giulianelli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jimenez de Asua, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00145793_v580_n10_p2512_Dekanty info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar FEBS Lett. 2006;580(10):2512-2516 Cyclin D1 ERK1-2 MAPK p38MAPK Prostaglandin F2α S-phase 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole cyclin D1 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 prostaglandin F2 alpha retinoblastoma protein cyclin D1 mitogen activated protein kinase prostaglandin F2 alpha animal cell article cell cycle S phase cell line cell proliferation controlled study DNA synthesis enzyme activation enzyme induction enzyme inhibition gene expression mouse nonhuman priority journal protein expression protein phosphorylation signal transduction animal cell division cell strain 3T3 DNA replication drug effect genetics metabolism polyacrylamide gel electrophoresis Western blotting Animalia Animals Blotting, Western Cell Division Cyclin D1 Dinoprost DNA Replication Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Mitogen-Activated Protein Kinases Swiss 3T3 Cells Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v580_n10_p2512_Dekanty_oai |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-145 |
| collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
| topic |
Cyclin D1 ERK1-2 MAPK p38MAPK Prostaglandin F2α S-phase 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole cyclin D1 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 prostaglandin F2 alpha retinoblastoma protein cyclin D1 mitogen activated protein kinase prostaglandin F2 alpha animal cell article cell cycle S phase cell line cell proliferation controlled study DNA synthesis enzyme activation enzyme induction enzyme inhibition gene expression mouse nonhuman priority journal protein expression protein phosphorylation signal transduction animal cell division cell strain 3T3 DNA replication drug effect genetics metabolism polyacrylamide gel electrophoresis Western blotting Animalia Animals Blotting, Western Cell Division Cyclin D1 Dinoprost DNA Replication Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Mitogen-Activated Protein Kinases Swiss 3T3 Cells |
| spellingShingle |
Cyclin D1 ERK1-2 MAPK p38MAPK Prostaglandin F2α S-phase 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole cyclin D1 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 prostaglandin F2 alpha retinoblastoma protein cyclin D1 mitogen activated protein kinase prostaglandin F2 alpha animal cell article cell cycle S phase cell line cell proliferation controlled study DNA synthesis enzyme activation enzyme induction enzyme inhibition gene expression mouse nonhuman priority journal protein expression protein phosphorylation signal transduction animal cell division cell strain 3T3 DNA replication drug effect genetics metabolism polyacrylamide gel electrophoresis Western blotting Animalia Animals Blotting, Western Cell Division Cyclin D1 Dinoprost DNA Replication Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Mitogen-Activated Protein Kinases Swiss 3T3 Cells Dekanty, A. Giulianelli, S. Coso, O.A. Rudland, P.S. Jimenez de Asua, L. Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α |
| topic_facet |
Cyclin D1 ERK1-2 MAPK p38MAPK Prostaglandin F2α S-phase 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole cyclin D1 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase p38 prostaglandin F2 alpha retinoblastoma protein cyclin D1 mitogen activated protein kinase prostaglandin F2 alpha animal cell article cell cycle S phase cell line cell proliferation controlled study DNA synthesis enzyme activation enzyme induction enzyme inhibition gene expression mouse nonhuman priority journal protein expression protein phosphorylation signal transduction animal cell division cell strain 3T3 DNA replication drug effect genetics metabolism polyacrylamide gel electrophoresis Western blotting Animalia Animals Blotting, Western Cell Division Cyclin D1 Dinoprost DNA Replication Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Mitogen-Activated Protein Kinases Swiss 3T3 Cells |
| description |
Prostaglandin F2α (PGF2α) induces cyclin D1 expression and DNA synthesis in Swiss 3T3 cells. In order to assess which signaling mechanisms are implicated in these processes, we have used both a pharmacological approach and interfering mutants. We demonstrate that PGF2α induces extracellular-signal-regulated kinase (ERK1-2) and p38MAPK activation, and inhibition of any of these signaling pathways completely blocks PGF2α-stimulated DNA synthesis. We also show that ERK1-2, but not p38MAPK activation is required to induce cyclin D1 expression, strongly suggesting that the concerted action of cyclin D1 gene expression and other events are required to induce complete phosphorylation of retinoblastoma protein and S-phase entry in response to PGF2α. © 2006. |
| format |
Artículo Artículo publishedVersion |
| author |
Dekanty, A. Giulianelli, S. Coso, O.A. Rudland, P.S. Jimenez de Asua, L. |
| author_facet |
Dekanty, A. Giulianelli, S. Coso, O.A. Rudland, P.S. Jimenez de Asua, L. |
| author_sort |
Dekanty, A. |
| title |
Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α |
| title_short |
Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α |
| title_full |
Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α |
| title_fullStr |
Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α |
| title_full_unstemmed |
Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α |
| title_sort |
differential involvement of erk1-2 and p38mapk activation on swiss 3t3 cell proliferation induced by prostaglandin f2α |
| publishDate |
2006 |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v580_n10_p2512_Dekanty https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00145793_v580_n10_p2512_Dekanty_oai |
| work_keys_str_mv |
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| _version_ |
1809357055690014720 |