Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth

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We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathoge...

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Autores principales: Prado-Acosta, M., Ruzal, S.M., Allievi, M.C., Palomino, M.M., Rivas, C.S.
Formato: Artículo publishedVersion
Publicado: 2010
Materias:
Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacteria (microorganisms)
Negibacteria
Posibacteria
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v76_n3_p974_PradoAcosta_oai
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Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
id I28-R145-paper_00992240_v76_n3_p974_PradoAcosta_oai
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spelling I28-R145-paper_00992240_v76_n3_p974_PradoAcosta_oai2024-08-16 Prado-Acosta, M. Ruzal, S.M. Allievi, M.C. Palomino, M.M. Rivas, C.S. 2010 We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Appl. Environ. Microbiol. 2010;76(3):974-977 Bacilli Bacillus cereus Bacteriology Cytology Hydrolases Salmonella Bacterial growth Gram-positive bacterium Hydrolase activities Lactobacillus acidophilus Proton-motive forces Salmonella enterica Staphylococcus aureus Synergistic effect Antibiotics bacterium enzyme activity growth rate membrane surface layer synergism Bacillus cereus Bacteria (microorganisms) Lactobacillus acidophilus Negibacteria Posibacteria Salmonella enterica Staphylococcus aureus Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v76_n3_p974_PradoAcosta_oai
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-145
collection Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
spellingShingle Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
Prado-Acosta, M.
Ruzal, S.M.
Allievi, M.C.
Palomino, M.M.
Rivas, C.S.
Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
topic_facet Bacilli
Bacillus cereus
Bacteriology
Cytology
Hydrolases
Salmonella
Bacterial growth
Gram-positive bacterium
Hydrolase activities
Lactobacillus acidophilus
Proton-motive forces
Salmonella enterica
Staphylococcus aureus
Synergistic effect
Antibiotics
bacterium
enzyme activity
growth rate
membrane
surface layer
synergism
Bacillus cereus
Bacteria (microorganisms)
Lactobacillus acidophilus
Negibacteria
Posibacteria
Salmonella enterica
Staphylococcus aureus
description We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
format Artículo
Artículo
publishedVersion
author Prado-Acosta, M.
Ruzal, S.M.
Allievi, M.C.
Palomino, M.M.
Rivas, C.S.
author_facet Prado-Acosta, M.
Ruzal, S.M.
Allievi, M.C.
Palomino, M.M.
Rivas, C.S.
author_sort Prado-Acosta, M.
title Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_short Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_full Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_fullStr Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_full_unstemmed Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
title_sort synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
publishDate 2010
url http://hdl.handle.net/20.500.12110/paper_00992240_v76_n3_p974_PradoAcosta
https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00992240_v76_n3_p974_PradoAcosta_oai
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AT ruzalsm synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth
AT allievimc synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth
AT palominomm synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth
AT rivascs synergisticeffectsofthelactobacillusacidophilussurfacelayerandnisinonbacterialgrowth
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